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This introduction collects 17 innovative approaches to engineer novel and improved proteins for diverse applications in biotechnology, chemistry, bioanalytics and medicine. As such, key developments covered in this reference and handbook include de novo enzyme design, cofactor design and metalloenzymes, extremophile proteins, and chemically resistant proteins for industrial processes. The editors integrate academic innovations and industrial applications so as to arrive at a balanced view of this multi-faceted topic.Throughout, the content is chosen to complement and extend the previously published two-volume handbook by the same editors, resultingin a superb overview of this burgeoning field.
Sommario
PREFACE DIRIGENT EFFECTS IN BIOCATALYSIS Introduction Dirigent Proteins Solvents and Unconventional Reaction Media Structure and Folding Structured and Unstructured Domains Isozymes, Moonlighting Proteins, and Promiscuity: Supertalented Enzymes Conclusions PROTEIN ENGINEERING GUIDED BY NATURAL DIVERSITY ApproachesProtocols Future Directions Conclusions PROTEIN ENGINEERING USING EUKARYOTIC EXPRESSION SYSTEMS Introduction Eukaryotic Expression Systems Conclusions PROTEIN ENGINEERING IN MICRODROPLETS IntroductionDroplet Formats Perspectives FOLDING AND DYNAMICS OF ENGINEERED PROTEINS IntroductionProof-of-Principle Protein Designs Proteins Designed for Function Conclusions and Outlook ENGINEERING PROTEIN STABILITY Introduction Power and Scope of Protein Engineering to Enhance Stability Measurement of a Protein's Kinetic Stability Developments in Protein Stabilization ENZYMES FROM THERMOPHILIC ORGANISMS Introduction Hyperthermophiles Enzymes from Thermophiles and Their Reactions Production of Proteins from (Hyper)Thermophiles Protein Engineering of Thermophilic Proteins Cell Engineering in Hyperthermophiles Future Perspectives ENZYME ENGINEERING BY COFACTOR REDESIGN Introduction Natural Cofactors: Types, Occurrence, and Chemistry Inorganic Cofactors Organic Cofactors Redox Cofactors Concluding Remarks BIOCATALYST IDENTIFICATION BY ANAEROBIC HIGH-THROUGHPUT SCREENING OF ENZYME LIBRARIES AND ANAEROBIC MICROORGANISMS Introduction Oxygen-Sensitive Biocatalysts Biocatalytic Potential of Oxygen-Sensitive Enzymes and Microorganisms Anaerobic High-Throughput Screening Conclusions and Outlook ORGANOMETALLIC CHEMISTRY IN PROTEIN SCAFFOLDS Introduction Protocol/Practical Considerations Goals Summary ENGINEERING PROTEASE SPECIFICITY Introduction Protocol and Practical Considerations Concepts, Challenges, and Visions on Future Developments POLYMERASE ENGINEERING: FROM PCR AND SEQUENCING TO SYNTHETIC BIOLOGY Introduction PCR Sequencing Polymerase Engineering StrategiesSynthetic Informational Polymers ENGINEERING GLYCOSYLTRANSFERASES Introduction to Glycosyltransferases Glycosyltransferase Sequence, Structure, and Mechanism Examples of Glycosyltransferase Engineering Practical Considerations for Screening Glycosyltransferases Future Directions and Outlook PROTEIN ENGINEERING OF CYTOCHROME P450 MONOOXYGENASESCytochrome P450 Monooxygenases Engineering of P450 Monooxygenases Conclusions PROGRESS AND CHALLENGES IN COMPUTATIONAL PROTEIN DESIGN Introduction The Technique of Computational Protein Design Protein Core Redesign, Structural Alterations, and Thermostabilization Computational Enzyme Design Computational Protein - Protein Interface Design Computational Redesign of DNA Binding and Specificity Conclusions SIMULATION OF ENZYMES IN ORGANIC SOLVENTSEnzymes in Organic Solvents Molecular Dynamics Simulations of Proteins and Solvents The Role of the Solvent Simulation of Protein Structure and FlexibilitySimulation of Catalytic Activity and Enantioselectivity Simulation of Solvent-Induced Conformational Transitions Challenges The Future of Biocatalyst Design ENGINEERING OF PROTEIN TUNNELS: THE KEYHOLE - LOCK - KEY MODEL FOR CATALYSIS BY ENZYMES WITH BURIED ACTIVE SITES Traditional Models of Enzymatic Catalysis Definition of the Keyhole - Lock - Key Model Robustness and Applicability of the Keyhole - Lock - Key Model Evolutionary and Functional Implications of the Keyhole - Lock - Key Model Engineering Implications of the Keyhole - Lock - Key Model Software Tools for the Rational Engineering of Keyholes Case Studies with Haloalkane Dehalogenases Conclusions INDEX
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Stefan Lutz holds a B. S. degree from the Zurich University of Applied Sciences (Switzerland), and a M.S. degree from the University of Teesside (UK). He then obtained a Ph.D. from the University of Florida and spent three years as a Postdoc with Stephen Benkovic at Pennsylvania State University under a fellowship of the Swiss National Science Foundation. Since 2002 he has been a Chemistry professor at Emory University in Atlanta, Georgia (USA). The research in the Lutz laboratory focuses on the structure-function relationship of proteins through combinatorial protein engineering and design.
Uwe Bornscheuer studied Chemistry at the University of Hannover (Germany), where he obtained a Ph. D. at the Institute of Technical Chemistry. He then spent a postdoctoral year at the University of Nagoya, Japan, before returning to Germany to join the Institute of Technical Biochemistry at the University of Stuttgart. Since 1999 he has been Professor for Biotechnology and Enzyme Catalysis at the University of Greifswald. His main research interest is the application of engineered enzymes in the synthesis of optically active compounds and in lipid modification.
Riassunto
This introduction collects 17 innovative approaches to engineer novel and improved proteins for diverse applications in biotechnology, chemistry, bioanalytics and medicine. As such, key developments covered in this reference and handbook include de novo enzyme design, cofactor design and metalloenzymes, extremophile proteins, and chemically resistant proteins for industrial processes. The editors integrate academic innovations and industrial applications so as to arrive at a balanced view of this multi-faceted topic.
Throughout, the content is chosen to complement and extend the previously published two-volume handbook by the same editors, resulting
in a superb overview of this burgeoning field.
