Lipidomics - Technologies and Applications

Focusing on the practical applications, this user-oriented guide presents current technologies and strategies for systems-level lipidanalysis, going beyond basic research to concentrate on commercial uses of lipidomics in biomarker and diagnostic development, as wellas within pharmaceutical drug discovery and development. The editor and authors have experience of the most recent analytical instruments and techniques, allowing them to provide here first-hand practical experience for newcomers to the field. The first half of the book covers current methodologies, ranging from global to targeted lipidomics and shotgun approaches, while the second part discusses the role of lipidomics in biomedical and pharmaceutical research, covering such diverse fields as inflammation, metabolic syndrome, cardiovascular and neurological disease. Both small and large-scale, high-throughput approaches are discussed, resulting in an invaluable source for academic and industrial research and development.

Sommario

PREFACELIPIDOMICS PERSPECTIVE: FROM MOLECULAR LIPIDOMICS TO VALIDATED CLINICAL DIAGNOSTICS IntroductionHierarchical Categorization of the Analytical Lipid OutputsThe Type of Lipid Information Delivers Different Biological KnowledgeUntying New Biological Evidences through Molecular Lipidomic ApplicationsMolecular Lipidomics Approaches Clinical DiagnosticsCurrent Roadblocks in LipidomicsConclusionsLIPIDS IN CELLSIntroductionBasis of Cellular Lipid DistributionLipid Distribution by Nonvesicular Routes Lipids in Different Cell Types Functional Implications of Membrane Lipid CompositionOutlook: Collectives and Phase SeparationHIGH-THROUGHPUT MOLECULAR LIPIDOMICS Introduction Lipid Diversity Function of Molecular Lipids Automated Sample Preparation Different Approaches to Molecular LipidomicsData Processing and EvaluationLipidomic Workflows Conclusions and Future Perspectives MULTIDIMENSIONAL MASS SPECTROMETRY-BASED SHOTGUN LIPIDOMICS IntroductionMultidimensional Mass Spectrometry-Based Shotgun Lipidomics Application of Multidimensional Mass Spectrometry-Based Shotgun Lipidomics for Lipidomic AnalysisConclusionsTARGETED LIPIDOMICS: SPHINGOLIPIDOMICSIntroduction Sphingolipids Description and Nomenclature Sphingolipids Analysis via Targeted LC-MS/MS Applications of Sphingolipidomics in Biology and Disease ConclusionsSTRUCTURAL LIPIDOMICSIntroduction Lipid Structure Structural Analysis of Lipids by Mass Spectrometry sn PositionDouble Bond PositionDouble Bond Stereochemistry Conclusions IMAGING LIPIDS IN TISSUES BY MATRIX-ASSISTED LASER DESORPTION/IONIZATION MASS SPECTROMETRY Introduction Sample Preparation Matrix Instrumentation Data Processing Conclusions LIPID INFORMATICS: FROM A MASS SPECTRUM TO INTERACTOMICS Introduction Lipid Nomenclature Basic Properties of Lipid Mass Spectrometric DataData Processing Lipidomic Data Mining and Visualization Lipidomic Data Integration Conclusions and Future PerspectivesLIPIDS IN HUMAN DISEASES Introduction Obesity Dyslipidemia Diabetes Cardiovascular DisordersHereditary Sensory NeuropathyNeurodegenerationCancer Lysosomal Storage Disorders Cystic Fibrosis Anti-Inflammatory Lipid Mediators ConclusionsLIPIDOMICS IN LIPOPROTEIN BIOLOGY Introduction Metabolism of Lipoproteins Lipoproteinomics in Normolipidemic Subjects Altered Lipoproteinomics in DyslipidemiaConclusionsMEDIATOR LIPIDOMICS IN INFLAMMATION RESEARCH IntroductionPUFA-Derived Lipid Mediators: Formation and Action LC-ESI-MS/MS-Based Lipidomics Mediator Lipidomics in Inflammation and ResolutionConclusion and Future PerspectiveLIPIDOMICS FOR ELUCIDATION OF METABOLIC SYNDROME AND RELATED LIPID METABOLIC DISORDER Introduction Basic Strategy of Lipidomics for Elucidating Metabolic Changes of Lipids at the Level of their Molecular Species in Metabolic Syndrome and Related DiseasesAnalytical Systems by Mass Spectrometry in Lipidomics Lipidomic Data Processing üAnalysis of Lipids as Markers of Metabolic Syndrome Direct Detection of Lipid Molecular Species in Specific Tissue Domains by Disease-Specific Changes ConclusionsLIPIDOMICS IN ATHEROSCLEROTIC VASCULAR DISEASE IntroductionLipids and Atherosclerotic Vascular Disease Diagnostics and Treatment Conclusions LIPID METABOLISM IN NEURODEGENERATIVE DISEASES IntroductionParkinson?s Disease Conclusions THE TUMOR MITOCHONDRIAL LIPIDOME AND RESPIRATORY BIOENERGETIC INSUFFICIENCYIntroduction Cardiolipin and Electron Transport Chain Abnormalities in Mouse Brain Tumor MitochondriaComplicating Influence of the in vitro Growth Environment on Cardiolipin Composition and Energy MetabolismBioinformatic Methods to Interpret Alterations in the Mitochondrial LipidomeConclusionsLIPIDOMICS FOR PHARMACEUTICAL RESEARCHIntroductionBiomarkers for Pharmaceutical ResearchStrategy for Biomarker Discovery Conclusions INDEX

Info autore

Kim Ekroos currently heads the bioanalytics division at Zora Biosciences in Espoo (Finland). He holds a Ph.D. from the Technical University of Dresden (Germany) and has conducted research in the group of Professor Kai Simons and Dr. Andrej Shevchenko at the Max-Planck Institute of Molecular Cell Biology and Genetics in Dresden. Dr. Ekroos has also worked at the European Molecular Biology Laboratory in Heidelberg (Germany). He has made major contributions to the advancement of basic research on lipids and their study with advanced mass
spectroscopy methods and software tools. In addition, he has pharmaceutical industry experience from Astra Zeneca where he spent three years successfully developing and utilizing high-throughput molecular lipidomics methods. Today he is focusing on applied molecular lipidomics for unscrambling the mechanistic details by which alterations in tissue-specific lipid metabolism are directly linked to the etiology of lipid-mediated disorders for the benefit of basic science, drug target and lipid biomarker discovery, and development of clinical diagnostics.

Riassunto

Focusing on the practical applications, this user-oriented guide presents current technologies and strategies for systems-level lipid
analysis, going beyond basic research to concentrate on commercial uses of lipidomics in biomarker and diagnostic development, as well
as within pharmaceutical drug discovery and development. The editor and authors have experience of the most recent analytical instruments and techniques, allowing them to provide here first-hand practical experience for newcomers to the field. The first half of the book covers current methodologies, ranging from global to targeted lipidomics and shotgun approaches, while the second part discusses the role of lipidomics in biomedical and pharmaceutical research, covering such diverse fields as inflammation, metabolic syndrome, cardiovascular and neurological disease. Both small and large-scale, high-throughput approaches are discussed, resulting in an invaluable source for academic and industrial research and development.

Dettagli sul prodotto

• Autori: Kim Ekroos
• Con la collaborazione di: Ki Ekroos (Editore), Kim Ekroos (Editore)
• Editore: Wiley-VCH
• Lingue: Inglese
• Formato: Copertina rigida
• Pubblicazione: 01.10.2012
• EAN: 9783527330980
• ISBN: 978-3-527-33098-0
• Pagine: 336
• Peso: 920 g
• Illustrazioni: 61 SW-Abb., 11 Farbabb., 10 Tabellen
• Categorie: Scienze naturali, medicina, informatica, tecnica > Chimica > Chimica organica; Chemie, Biochemie, Analytische Chemie, Life Sciences, genom, Lipide, Biowissenschaften, Chemische Biologie, chemistry, Analytical Chemistry, Biochemie u. Chemische Biologie, Biochemistry (Chemical Biology), Genomforschung u. Proteomik, Molecular Techniques, Molekulare Methoden, Genomics & Proteomics