Fr. 179.00

Chromatin Immunoprecipitation Assays - Methods and Protocols

Inglese · Tascabile

Spedizione di solito entro 6 a 7 settimane

Descrizione

Ulteriori informazioni

Over the past twenty years, the development of chromatin immunoprecipitation, or ChIP, assays has immensely enhanced the biological significance of the multifaceted DNA-binding proteins. In Chromatin Immunoprecipitation Assays: Methods and Protocols, researchers deeply involved in the development and improvement of the field provide cutting-edge protocols devoted to the most recent progress in ChIP and related subjects. The thorough chapters involve topics such as the characterization of ChIP antibodies, ChIP methods for small cell numbers, fast ChIP protocols, assays adapted to various species and cell types, as well as several strategies for the analysis of genome-wide datasets, while also extending a bit beyond ChIP assays to cover immunoprecipitation-based DNA methylation analyses, determination of spatial chromatin organization of large genomic regions, and RNA immunoprecipitation. Written in the highly successful Methods in Molecular Biology(TM) series format, chapters contain brief introductions to their respective subjects, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes on troubleshooting and avoiding known pitfalls.
Authoritative and easy-to-use, Chromatin Immunoprecipitation Assays: Methods and Protocols is a timely guide for all those who wish to further the development of this powerful tool and continue delving into the incredible complexity of the cell's biology.

Sommario

The State-of-the-Art of Chromatin Immunoprecipitation.- Characterization and Quality Control of Antibodies Used in ChIP Assays.- The Fast Chromatin Immunoprecipitation Method.- ?ChIP: Chromatin Immunoprecipitation for Small Cell Numbers.- Fish'n ChIPs: Chromatin Immunoprecipitation in the Zebrafish Embryo.- Epitope Tagging of Endogenous Proteins for Genome-Wide Chromatin Immunoprecipitation Analysis.- Flow Cytometric and Laser Scanning Microscopic Approaches in Epigenetics Research.- Serial Analysis of Binding Elements for Transcription Factors.- Modeling and Analysis of ChIP-Chip Experiments.- Use of SNP-Arrays for ChIP Assays: Computational Aspects.- DamID: A Methylation-Based Chromatin Profiling Approach.- Chromosome Conformation Capture (from 3C to 5C) and Its ChIP-Based Modification.- Determining Spatial Chromatin Organization of Large Genomic Regions Using 5C Technology.- Analysis of Nascent RNA Transcripts by Chromatin RNA Immunoprecipitation.- Methyl DNA Immunoprecipitation.- Immunoprecipitation of Methylated DNA.- Erratum to: Chromosome Conformation Capture (from 3C to 5C) and Its ChIP-Based Modification.

Riassunto

Over the past twenty years, the development of chromatin immunoprecipitation, or ChIP, assays has immensely enhanced the biological significance of the multifaceted DNA-binding proteins. In Chromatin Immunoprecipitation Assays: Methods and Protocols, researchers deeply involved in the development and improvement of the field provide cutting-edge protocols devoted to the most recent progress in ChIP and related subjects. The thorough chapters involve topics such as the characterization of ChIP antibodies, ChIP methods for small cell numbers, fast ChIP protocols, assays adapted to various species and cell types, as well as several strategies for the analysis of genome-wide datasets, while also extending a bit beyond ChIP assays to cover immunoprecipitation-based DNA methylation analyses, determination of spatial chromatin organization of large genomic regions, and RNA immunoprecipitation. Written in the highly successful Methods in Molecular Biology™ series format, chapters contain brief introductions to their respective subjects, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes on troubleshooting and avoiding known pitfalls.

Authoritative and easy-to-use, Chromatin Immunoprecipitation Assays: Methods and Protocols is a timely guide for all those who wish to further the development of this powerful tool and continue delving into the incredible complexity of the cell’s biology.

Testo aggiuntivo

From the reviews:
“This is a very useful book for researchers who utilise chromatin Immunoprecipitation (ChIP). … In conclusion, the chapters in this book describe the methods in detail and in general they have reasonable bibliographies. This book is a very useful acquisition for researchers who are embarking upon research that employs ChIP and equally for those researchers who are already undertaking it … .”­­­ (Ray Waters, Human Genetics, Vol. 127, March, 2010)

Relazione

From the reviews:
"This is a very useful book for researchers who utilise chromatin Immunoprecipitation (ChIP). ... In conclusion, the chapters in this book describe the methods in detail and in general they have reasonable bibliographies. This book is a very useful acquisition for researchers who are embarking upon research that employs ChIP and equally for those researchers who are already undertaking it ... ." (Ray Waters, Human Genetics, Vol. 127, March, 2010)

Dettagli sul prodotto

Con la collaborazione di Philipp Collas (Editore), Philippe Collas (Editore)
Editore Springer, Berlin
 
Lingue Inglese
Formato Tascabile
Pubblicazione 01.01.2014
 
EAN 9781617797507
ISBN 978-1-61779-750-7
Pagine 268
Dimensioni 195 mm x 261 mm x 19 mm
Peso 592 g
Illustrazioni X, 268 p. 47 illus.
Serie Methods in Molecular Biology
Methods in Molecular Biology
Categorie Scienze naturali, medicina, informatica, tecnica > Biologia > Biochimica, biofisica

B, Life Sciences, biochemistry, bioinformatics, Human Genetics, Biomedical and Life Sciences, Medical Genetics, Biochemistry, general, Microarrays, Computational biology / bioinformatics

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