Proteome Research: Two-Dimensional Gel Electrophoresis and Identification Methods

Two-dimensional electrophoresis is the central methodology in proteome research, and the state of the art is described in detail in this text, together with extensive coverage of the detection methods available. Sufficient detail is given to allow the readers to apply these technologies to their own particular requirements.

Sommario

Solubilization of proteins in 2D electrophoresis.- 2D electrophoresis with carrier ampholytes.- 2D electrophoresis with immobilized pH gradients.- Detection of proteins on 2D electrophoresis gels.- Blotting and immunoaffinity identification of 2-DE separated proteins.- Identification of proteins by amino acid composition after acid hydrolysis.- Identification by amino acid composition obtained from labeling.- Identification of proteins by amino acid sequencing.- Identification of proteins by mass spectrometry.- Mass spectrometry of intact proteins from two-dimensional PAGE.

Dettagli sul prodotto

• Con la collaborazione di: Rabilloud (Editore), T Rabilloud (Editore), T. Rabilloud (Editore)
• Editore: Springer, Berlin
• Lingue: Inglese
• Formato: Tascabile
• Pubblicazione: 05.02.2001
• EAN: 9783540657927
• ISBN: 978-3-540-65792-7
• Pagine: 248
• Peso: 424 g
• Illustrazioni: XVI, 248 p.
• Serie: Principles and Practice; Principles and Practice
• Categoria: Scienze naturali, medicina, informatica, tecnica > Biologia > Biochimica, biofisica