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Informationen zum Autor Dr David R. Poyner, University of Aston, Birmingham, UK; School of Life and Health Sciences, Aston University, Birmingham, UK. Professor?Mark Wheatley, University of Birmingham, UK. Klappentext G Protein Coupled Receptors (GPCRs) are not only the largest protein family in the human genome but are also the single biggest target for therapeutic agents. Research into GPCRs is therefore growing at a fast pace and the range of techniques that can be applied to GPCRs is vast and continues to grow. This book provides an invaluable bench-side guide into the best and most up-to-date techniques for current and future research on GPCRs. With contributions from leading international authorities, GPCR Essential Methods equips readers with clear and detailed protocols for both well-known and up-and-coming techniques along with hints and tips for success. All the methods have been tried and tested by leading international research labs and are presented in easy-to-follow stages along with a useful overview of each technique. This book will be of interest to researchers in molecular biology, biochemistry, pharmacology and will be a useful resource for graduate students. Highlights the best and most up-to-date techniques for working on GPCRs from the vast range currently in use Explains clearly and precisely how to carry out selected techniques in addition to background information on the various approaches Chapters are written by leading international authorities in the field and cover both new and well-known, tried and tested methods for working with GPCRs An essential laboratory manual for researchers at all levels Zusammenfassung Provides an invaluable bench-side guide into the best and most up-to-date techniques for current and future research on GPCRs. Includes contributions from leading international authorities. Equips readers with clear and detailed protocols for both well-known and up-and-coming techniques along with hints and tips for success. Inhaltsverzeichnis Preface. Contributors. 1 Measurement of Ligand-G-Protein-coupled Receptor Interactions ( Katie Leach, Celine Valant, Patrick M. Sexton and Arthur Christopoulos ). 1.1 Introduction. 1.2 Methods and Approaches. References. 2 Second Messenger Assays for G-Protein-coupled Receptors: cAMP, Ca2+, Inositol Phosphates, ERK1/2 ( Karen J. Gregory, Patrick M. Sexton, Arthur Christopoulos and Caroline A. Hick ). 2.1 Introduction. 2.2 Methods and Approaches. 2.3 Troubleshooting. References. 3 Use of the [35S]GTP ¿ S Binding Assay to Determine Ligand Efficacy at G-protein-coupled Receptors ( Elodie Kara and Philip G. Strange ). 3.1 Introduction. 3.2 Methods and Approaches. 3.3 Troubleshooting. Acknowledgements. References. 4 Quantitative Imaging of Receptor Trafficking ( Andy R. James, Takeo Awaji, F. Anne Stephenson and Nicholas A. Hartell ). 4.1 Introduction. 4.2 Methods and Approaches. 4.3 Troubleshooting. References. 5 Production of Recombinant G-Protein-coupled Receptor in Yeast for Structural and Functional Analysis ( Richard A.J. Darby, Mohammed Jamshad, Ljuban Grgic, William J. Holmes and Roslyn M. Bill ). 5.1 Introduction. 5.2 Methods and Approaches. 5.3 Troubleshooting. References. 6 Monitoring GPCR-Protein Complexes Using Bioluminescence Resonance Energy Transfer ( Werner C. Jaeger, Kevin D.G. Pfleger and Karin A. Eidne ). 6.1 Introduction. 6.2 Methods and Approaches. 6.3 Troubleshooting. References. 7 Using Intramolecular Fluorescence Resonance Energy Transfer to Study Receptor Conformation ( Cornelius Krasel and Carsten Hoffmann ).
