Read more
Informationen zum Autor Editors Banafshé Larijani Head of Cell Biophysics Laboratory, Cancer Research UK, London Research Institute Colin A Rosser Director of Studies, Rye St Antony School, Oxford Rudiger Woscholski Division of Cell and Molecular Biology, Faculty of Natural Sciences and Chemical Biology Centre (CBC), Imperia/ College, London Klappentext Chemical Biology Techniques and Applications Written by a team of international researchers and teachers at the cutting edge of chemical biology research, this book provides an exciting, comprehensive introduction to a wide range of chemical and physical techniques with applications in areas as diverse as molecular biology, signal transduction, drug discovery and medicine. Techniques include; Cryo-electron microscopy, atomic force microscopy, differential scanning calorimetry in the study of lipid structures, membrane potentials and membrane probes, identification and quantification of lipids using mass spectroscopy, liquid state NMR, solid state NMR in biomembranes, molecular dynamics, two dimensional infra-red studies of biomolecules, single and two-photon fluorescence, optical tweezers, PET imaging and chemical genetics. key Features a unique guide to the rapidly evolving, interdisciplinary field of chemical biology. adopts a modular structure for maximum flexibility. addresses relevant, topical chemical biological questions throughout. includes stunning illustrations in full colour. associated website with PowerPoint slides of figures within the book. Chemical Biology: Techniques and Applications will provide an invaluable resource for final year undergraduate and post graduate bioscience and biomedical science students and pharmaceutical researchers with an interest in this fascinating, and ever changing field. Zusammenfassung Written by a team of international researchers and teachers at the cutting edge of chemical biology research, this book provides an exciting, comprehensive introduction to a wide range of chemical and physical techniques with applications in areas as diverse as molecular biology, signal transduction, drug discovery and medicine. Inhaltsverzeichnis Preface. List of Contributors. 1. Introduction. 1.1 Chemical biology - the present. 1.2 Chemical biology - the past. 1.3 Chemical biology - the future. 1.4 Chemical biology - mind the interdisciplinary gap. 1.5 An introduction to the following chapters. 2. Cryomicroscopy. 2.1 The need for (electron) microscopy. 2.2 Development of cryomicroscopy. 2.3 Sample-electron interaction. 2.4 Contrast in negatively stained and cryo preparations. 2.5 Image formation. 2.6 Image analysis. 2.7 Software used in the analysis of electron micrographs. 2.8 Examples. 2.9 Conclusions. 3. Atomic force microscopy: applications in biology. 3.1 A brief history of microscopy. 3.2 The scanning pribe microscope revolution. 3.3 The workings of an AFM instrument. 3.4 Imaging biological molecules with force. 3.5 Factors influencing image quality. 3.6 Biological applications of AFM and recent developments. 3.7 Conclusions and future directions. 4. Differential scanning calorimetry in the study of lipid structures. 4.1 Introduction. 4.2 Membranes, lipids and lipid phases. 4.3 Heat exchanges and calorimetry. 4.4 Phase transitions in pure lipid-water systems. 4.5 Selected examples of transitions in lipid mixtures. 4.6 Complex systems: lipid-protein mixtures and cell membranes. 4.7 Conclusion. 5. Membrane potentials and membrane probes. 5.1 Introduction: biological membranes; structure and electrical properties. 5.2 Pho...
