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Informationen zum Autor Philippe Soriano,is Professor of Cell, Developmental & Regenerative Biology and Professor of Oncological Sciences, Icahn School of Medicine at Mount Sinai, NY, USA Klappentext This volume is Part B of an update of volume 225, Guide to Techniques in Mouse Development, edited by P.M. Wassarman and M.L. DePamphilis and published in 1993. During the past 17 years many new technologies or methodologies have appeared for the study of mouse development, and this volume comprehensively covers these, including new techniques for the cryopreservation of gametes and embryos, production of transgenic and null (knockout) animals (use of ES cells), generation of conditional/inducible mutant animals, use of gene-trap mutagenesis, analysis of allele-specific expression, use of new reporter constructs, humanizing of transgenic animals, transcript profiling of mouse development, imaging of mouse development, and rederivation of animals and use of mouse genomics. Inhaltsverzeichnis Section I. Transgenesis 1. Lentivirus transgenesis2. Germline modification using mouse spermatogonial stem cells3. Embryonic in vivo electroporation in the mouse Section II. Transposons 4. Current applications of transposons in mouse genetics5. Functional genomics in the mouse using the Sleeping Beauty transposon system6. The use of DNA transposons for cancer gene discovery in mice Section III. Recombinases 7. A practical summary of site specific recombination, conditional mutagenesis, and tamoxifen induction of CreERT28. A recombineering pipeline to make conditional targeting constructs 9. Confirmation of recombination site functionality in gene targeting vectors using recombinase-expressing bacteria10.Genetic fate mapping using site-specific recombinases11. Mapping cell fate and function using recombinase-based intersectional strategies Section IV. Mutagenesis 12. Genome-wide forward genetic screens in mouse ES cells13. Gene trap mutagenesis in the mouse14. A wider context for gene trap mutagenesis 15. Mouse mutagenesis with the chemical supermutagen ENU16. Phenotype-driven mouse ENU mutagenesis screens17. Using ENU mutagenesis for phenotype-driven analysis of the mouse Section V. Gene Knockdowns 18. Exploration of self-renewal and pluripotency in ES cells using RNAi19. Transgenic RNAi applications in the mouse 20. Gene knockdown in the mouse through RNAi21. In vivo analysis of gene knock-down in tetracycline-inducible shRNA mice22. The power of reversibility: Regulating gene activities via tetracycline controlled transcription Section VI. Gene Expression Profiling 23. Gene expression profiling of mouse oocytes and preimplantation embryos24. Interrogating the transcriptome of oocytes and preimplantation embryos25. Gene expression profiling of mouse embryos with microarrays ...
List of contents
Section I. Transgenesis1. Lentivirus transgenesis2. Germline modification using mouse spermatogonial stem cells3. Embryonic in vivo electroporation in the mouse
Section II. Transposons4. Current applications of transposons in mouse genetics5. Functional genomics in the mouse using the Sleeping Beauty transposon system6. The use of DNA transposons for cancer gene discovery in mice
Section III. Recombinases7. A practical summary of site specific recombination, conditional mutagenesis, and tamoxifen induction of CreERT28. A recombineering pipeline to make conditional targeting constructs 9. Confirmation of recombination site functionality in gene targeting vectors using recombinase-expressing bacteria10.Genetic fate mapping using site-specific recombinases11. Mapping cell fate and function using recombinase-based intersectional strategies
Section IV. Mutagenesis12. Genome-wide forward genetic screens in mouse ES cells13. Gene trap mutagenesis in the mouse14. A wider context for gene trap mutagenesis 15. Mouse mutagenesis with the chemical supermutagen ENU16. Phenotype-driven mouse ENU mutagenesis screens17. Using ENU mutagenesis for phenotype-driven analysis of the mouse
Section V. Gene Knockdowns18. Exploration of self-renewal and pluripotency in ES cells using RNAi19. Transgenic RNAi applications in the mouse 20. Gene knockdown in the mouse through RNAi21. In vivo analysis of gene knock-down in tetracycline-inducible shRNA mice22. The power of reversibility: Regulating gene activities via tetracycline controlled transcription
Section VI. Gene Expression Profiling23. Gene expression profiling of mouse oocytes and preimplantation embryos24. Interrogating the transcriptome of oocytes and preimplantation embryos25. Gene expression profiling of mouse embryos with microarrays
