Cryo-Em Part a - Sample Preparation and Data Collection

Describes the instruments, samples, protocols, and analyses that belong to cryo-EM. This title emphasizes the relatedness of the ideas, instrumentation, and methods underlying cryo-EM approaches which allow practitioners to easily move between them.

List of contents

Historical perspective
3D reconstruction from electron micrographs: a personal account of its development
David DeRosier

1. Preparation of 2-D crystals
Henning Stahlberg


2. Helical Crystallization of Soluble and Membrane Binding Proteins
Elizabeth M. Wilson-Kubalek, Christopher Arthur, Joshua S. Chappie


3. Plunge Freezing for Electron Cryomicroscopy
Alasdair W. McDowall, Megan J. Dobro, Linda A. Melanson, Grant J. Jensen


4. A Practical Guide to the Use of Monolayer Purification and Affinity Grids
Thomas Walz, Deborah F. Kelly, Danijela Dukovski


5. GraFix: Stabilization of fragile macromolecular complexes for single particle cryo-EM
Holger Stark


6. Cryo-negative staining of macromolecular assemblies
Sacha De Carlo, Holger Stark


7. Liposomes on a streptavidin crystal: a system to study membrane proteins by cryo-EM
Fred J. Sigworth, Liguo Wang


8. Micromanipulator-Assisted Vitreous Cryosectioning and Sample Preparation by High-Pressure Freezing
Mark S. Ladinsky


9. Site-Specific Biomolecule Labeling with Gold Clusters
Christopher J. Ackerson, Richard D. Powell, James F. Hainfeld


10. How to Operate a Cryo-Electron Microscope
Huilin Li, Jingchuan Sun


11. Collecting electron crystallographic data of two-dimensional protein crystals
Thomas Walz, Richard K. Hite, Andreas D. Schenk, Zongli Li, Yifan Cheng


12. Automated Data Collection for Electron Microscopic Tomography
David Agard, Shawn Q. Zheng, J. W. Sedat


13. Correlated Light and Electron Cryomicroscopy
Grant J. Jensen, Ariane Briegel, Songye Chen, Abraham J. Kosterc, Jürgen M. Plitzkod, Cindi L. Schwartz


14. Phase Plates for Transmission Electron Microscopy
Radostin Danev, Kuniaki Nagayama


15. Radiation damage in electron cryomicroscopy

John L. Rubinstein, Lindsay A. Baker

Product details

• Authors: Grant Jenson, Grant (EDT) Jenson
• Assisted by: Grant Jensen (Editor), Grant J. Jensen (Editor)
• Publisher: ELSEVIER SCIENCE BV
• Languages: English
• Product format: Hardback
• Release: 12.11.2010, delayed
• EAN: 9780123749062
• ISBN: 978-0-12-374906-2
• No. of pages: 464
• Series: Academic Press; Methods in Enzymology; Methods in Enzymology
• Subjects: Natural sciences, medicine, IT, technology > Medicine > Clinical medicine; Microscopy, SCIENCE / Microscopes & Microscopy