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Informationen zum Autor University of Pennsylvania, Philadelphia, USA and Institut Curie, Paris, France Klappentext Microtubules in Cells is complemented by the companion volume by Correia that covers methods to study microtubules in vitro. The Microtubules in Cells volume includes chapters by experts around the world on many aspects of microtubule imaging in living and fixed cells; assays to study microtubule function in a wide array of model organisms and cultured cells; and high-resolution approaches to study the cytoskeleton. The authors share their years of experience, outlining potential pitfalls and critical factors to consider in experimental design, experimental implementation and data interpretation. Zusammenfassung Icludes chapters on many aspects of microtubule imaging in living and fixed cells; assays to study microtubule function in a wide array of model organisms and cultured cells; and! high resolution approaches to study of the cytoskeleton. Inhaltsverzeichnis Microtubule dynamics at the cell cortex probed by TIRF microscopy Ilya Grigoriev and Anna Akhmanova Microscopy methods for the study of centriole biogenesis and function in Drosophila Ana Rodrigues Martins, Pedro Machado, Giuliano Callaini and Monica Bettencourt-Dias Quality control in single molecule studies of kinesins and microtubule-associated proteins Gary J. Brouhard Imaging cilia in zebrafish Kimberly M. Jaffe, Stephan Y. Thiberge, Margaret E. Bisher and Rebecca D. Burdine Automatic Identification of Microtubules in Cellular Electron Tomography Daniyar Nurgaliev, Timur Gatanov and Daniel J. Needleman Imaging and analysis of the microtubule cytoskeleton in Giardia Scott C. Dawson and Susan A. House Kinetochore-microtubule dynamics and attachment stability Jennifer G. DeLuca Assessment of mitotic spindle phenotypes in Drosophila S2 cells Gohta Goshima Live cell imaging techniques for analyses of microtubules in Dictyostelium Matthias Samereier, Irene Meyer, Michael P. Koonce and Ralph Gräf Microtubule dynamics in dendritic spines Lukas C. Kapitein , Kah Wai Yau and Casper C. Hoogenraad Analysis of Microtubules in Budding Yeast Alexander Rauch, Elena Nazarova and Jackie Vogel Determination of Microtubule Dynamic Instability in Living Cells Kathy Kamath, Emin Oroudjev, and Mary Ann Jordan Modeling microtubule-mediated forces and centrosome positioning in Caenorhabditis elegans embryos Akatsuki Kimura1 and Shuichi Onami Cryo electron tomography of cellular microtubules Roman I. Koning Microtubule dynamics in plant cells Henrik Buschmann, Adrian Sambade, Edouard Pesquet, Grant Calder and Clive W. Lloyd Drosophila S2 cells as a model system to investigate mitotic spindle dynamics, architecture and function Sara Moutinho-Pereira, Irina Matos and Helder Maiato Protein micropatterns: a direct printing protocol using deep UVs Ammar Azioune, Nicolas Carpi, Manuel Théry and Matthieu Piel Optical trapping and laser ablation of microtubules in fission yeast Nicola Maghelli and Iva M. Tolic-Nørrelykke Melanophores for Microtubule dynamics and motility assays Kazuho Ikeda, Irina Semenova, Olga Zhapparova, and Vladimir Rodionov New and old reagents for fluorescent-protein tagging of microtubules in fission yeast: experimental and critical evaluation Hilary A. Snaith, Andreas Anders, Itaru Samejima and Kenneth E. Sawin Imaging of mitotic spindle dynamics in C. elegans embryos Mika Toya, Yumi Iida and Asako Sugimoto A fast microfluidic temperature control device for studying microtubule dynamics in fission yeast Guilhem Velve-Casquillas, Judite Costa, Frédérique Carlier-Grynkorn , Adeline Mayeux and Phong T. Tran Microtubule-dependent spatial organization of mitochondria in fission...
