Nuclear Mechanics Genome Regulation

Presents comprehensive chapters and background information for researchers working within the field of nuclear mechanics and genome regulation. This title offers both classic and advanced methods readily adaptable and designed to last the test of time. It is of interest to clinicians and scientists working in a wide range of fields.

List of contents

Section-A: Cell Nucleus: Organization and MechanoBiology

1. Fluorescence Fluctuation Microscopy to reveal 3D-Architecture and Function in the Cell Nucleus
Thorsten Lenser^1,2, Klaus Weisshart²_, Tobias Ulbricht³, Karolin Klement³, and Peter Hemmerich


2. Studying histone modifications and their genomic functions by employing chromatin immunoprecipitation and immunoblotting
Ranveer S. Jayani, Praveena L. Ramanujam and Sanjeev Galande


3. Dynamic organization of chromatin assembly & transcription factories in living cells
Bidisha Sinha, Dipanjan Bhattacharya, Deepak Kumar Sinha, Shefali Talwar,
Shovamayee Maharana, Soumya Gupta and G. V.Shivashankar


4. Manipulation & Isolation of Single Cells & Nuclei
Swee Jin Tan, Qingsen Li and Chwee Teck Lim


5. Beyond lamins: other structural components of the nucleoskeleton
Zhixia Zhong , Katherine L. Wilson and Kris Noel Dahl


6. Altered mechanical properties of the nucleus in disease
Maria Lucia Lombardi and Jan Lammerding


7. Theoretical concepts and models of cellular mechanosensing
Rumi De, Assaf Zemel and Samuel A. Safran
Section-B: Impact of Nuclear Mechanics on Function


8. Mechanical Induction of Gene Expression in Connective Tissue Cells
Mathew Chan, Boris Hinz, Christopher A. McCulloch


9. Physical plasticity of the Nucleus and its manipulation
Irena Ivanovska, Joe Swift, Takamasa Harada, J. David Pajerowski and Dennis E. Discher


10. Prestressed Nuclear Organization in Living Cells
Aprotim Mazumder, Roopa T. , Abhishek Kumar, K.Venkatesan Iyer,
Nisha M Ramdas and G. V.Shivashankar


11. Nanotopography/mechanical induction of stem-cell differentiation
Evelyn Yim


12. Mechanical Induction in Embryonic Development and Tumour Growth: Integrative Cues through Molecular to Multi-cellular Interplay, and Evolutionary Perspectives
Maria-Elena Fernandez-Sanchez, Fanny Serman, Padra Ahmadi and Emmanuel Farge


13. Informatics based analysis of mechanosignaling in the laminopathies
Frank P.L. Lai, Radfidah A. Mutalif, Siew Cheng Phua, and Colin L. Stewart


14. Autosomal Dominant Leukodystrophy caused by lamin B1 duplications: A clinical and molecular case study of altered nuclear function and disease

Quasar Saleem Padiath and Ying-Hui Fu

Product details

• Authors: G.v. Shivashankar
• Assisted by: G. V. Shivashankar (Editor), G.V. Shivashankar (Editor)
• Publisher: Academic Press London
• Languages: English
• Product format: Hardback
• Released: 13.07.2010
• EAN: 9780123810090
• ISBN: 978-0-12-381009-0
• No. of pages: 250
• Series: Academic Press; Methods in Cell Biology; Methods in Cell Biology
• Subjects: Natural sciences, medicine, IT, technology > Biology > General, dictionaries; SCIENCE / Life Sciences / Genetics & Genomics, Genetics (non-medical), Dna & Genome, SCIENCE / Bioinformatics, Computational biology / bioinformatics