Practical High-Performance Liquid Chromatography

Informationen zum Autor Veronika R. Meyer, Department of Biocompatible Materials, Switzerland Swiss Federal Laboratories for Materials Testing and Research, Switzerland. Klappentext Jump into the HPLC adventure!Three decades on from publication of the 1st German edition of Veronika Meyer's book on HPLC, this classic text remains one of the few titles available on general HPLC aimed at practitioners.New sections on the following topics have been included in this fifth edition:* Comparison of HPLC with capillary electrophoresis* How to obtain peak capacity* van Deemter curves and other coherences* Hydrophilic interaction chromatography* Method transfer* Comprehensive two-dimensional HPLC* Fast separations at 1000 bar* HPLC with superheated waterIn addition, two chapters on the instrument test and troubleshooting in the appendix have been updated and expanded by Bruno E. Lendi, and many details have been improved and numerous references added.A completely new chapter is presented on quality assurance covering:* Is it worth the effort?* Verification with a second method* Method validation* Standard operating procedures* Measurement uncertainty* Qualifications, instrument test, and system suitability test* The quest for qualityReviews of earlier editions"That this text is written by an expert in both the practice and teaching of HPLC is evident from the first paragraph....not only an enjoyable, fascinating and easy read, but a truly excellent text that has and will serve many teachers, students and practitioners very well." --The Analyst"...provides essential information on HPLC for LC practitioners in academia, industry, government, and research laboratories...a valuable introduction." - American Journal of Therapeutics Zusammenfassung This textbook aims to present the knowledge which is necessary for everyday HPLC measurements to be made, in a detailed and easy-to-follow manner. It contains a wealth of practical material and covers a wide range of subjects. Inhaltsverzeichnis Preface to the Fifth Edition. Important and Useful Equations for HPLC. 1 Introduction. 1.1 HPLC: A powerful separation method. 1.2 A first HPLC experiment. 1.3 Liquid chromatographic separation modes. 1.4 The HPLC instrument. 1.5 Safety in the HPLC laboratory. 1.6 Comparison between high-performance liquid chromatography and gas chromatography. 1.7 Comparison between high-performance liquid chromatography and capillary electrophoresis. 1.8 Units for pressure, length and viscosity. 1.9 Scientific journals. 1.10 Recommended books. 2 Theoretical Principles. 2.1 The chromatographic process. 2.2 Band broadening. 2.3 The chromatogram and its purport. 2.4 Graphical representation of peak pairs with different degree of resolution. 2.5 Factors affecting resolution. 2.6 Extra-column volumes (dead volumes). 2.7 Tailing. 2.8 Peak capacity and statistical resolution probability. 2.9 Effects of temperature in HPLC. 2.10 The limits of HPLC. 2.11 How to obtain peak capacity. 3 Pumps. 3.1 General requirements. 3.2 The short-stroke piston pump. 3.3 Maintenance and repair. 3.4 Other pump designs. 4 Preparation of Equipment up to Sample Injection. 4.1 Selection of the mobile phase. 4.2 Preparation of the mobile phase. 4.3 Gradient systems. 4.4 Capillary tubing. 4.5 Fittings. 4.6 Sample injectors. 4.7 Sample solution and sample volume. 5 Solvent Properties. 5.1 Table of organic solvents. 5.2 Solvent selectivity. 5.3 Miscibility. 5.4 Buffers. 5.5 Shelf life of mobile phases. 5.6 The mixing cross. 6 Detectors. 6.1 General. 6.2 UV detectors. ...

List of contents

Preface to the Fifth Edition.
 
Important and Useful Equations for HPLC.
 
1 Introduction.
 
1.1 HPLC: A powerful separation method.
 
1.2 A first HPLC experiment.
 
1.3 Liquid chromatographic separation modes.
 
1.4 The HPLC instrument.
 
1.5 Safety in the HPLC laboratory.
 
1.6 Comparison between high-performance liquid chromatography and gas chromatography.
 
1.7 Comparison between high-performance liquid chromatography and capillary electrophoresis.
 
1.8 Units for pressure, length and viscosity.
 
1.9 Scientific journals.
 
1.10 Recommended books.
 
2 Theoretical Principles.
 
2.1 The chromatographic process.
 
2.2 Band broadening.
 
2.3 The chromatogram and its purport.
 
2.4 Graphical representation of peak pairs with different degree of resolution.
 
2.5 Factors affecting resolution.
 
2.6 Extra-column volumes (dead volumes).
 
2.7 Tailing.
 
2.8 Peak capacity and statistical resolution probability.
 
2.9 Effects of temperature in HPLC.
 
2.10 The limits of HPLC.
 
2.11 How to obtain peak capacity.
 
3 Pumps.
 
3.1 General requirements.
 
3.2 The short-stroke piston pump.
 
3.3 Maintenance and repair.
 
3.4 Other pump designs.
 
4 Preparation of Equipment up to Sample Injection.
 
4.1 Selection of the mobile phase.
 
4.2 Preparation of the mobile phase.
 
4.3 Gradient systems.
 
4.4 Capillary tubing.
 
4.5 Fittings.
 
4.6 Sample injectors.
 
4.7 Sample solution and sample volume.
 
5 Solvent Properties.
 
5.1 Table of organic solvents.
 
5.2 Solvent selectivity.
 
5.3 Miscibility.
 
5.4 Buffers.
 
5.5 Shelf life of mobile phases.
 
5.6 The mixing cross.
 
6 Detectors.
 
6.1 General.
 
6.2 UV detectors.
 
6.3 Refractive index detectors.
 
6.4 Fluorescence detectors.
 
6.5 Electrochemical (amperometric) detectors.
 
6.6 Light-scattering detectors.
 
6.7 Other detectors.
 
6.8 Multiple detection.
 
6.9 Indirect detection.
 
6.10 Coupling with spectroscopy.
 
7 Columns and Stationary Phases.
 
7.1 Columns for HPLC.
 
7.2 Precolumns.
 
7.3 General properties of stationary phases.
 
7.4 Silica.
 
7.5 Chemically modified silica.
 
7.6 Styrene-divinylbenzene.
 
7.7 Some other stationary phases.
 
7.8 Column care and regeneration.
 
8 HPLC Column Tests.
 
8.1 Simple tests for HPLC columns.
 
8.2 Determination of particle size.
 
8.3 Determination of breakthrough time.
 
8.4 The test mixture.
 
8.5 Dimensionless parameters for HPLC column characterization.
 
8.6 The van Deemter equation from reduced parameters and its use in column diagnosis.
 
8.7 van Deemter curves and other coherences.
 
8.8 Diffusion coefficients.
 
9 Adsorption Chromatography: Normal-Phase Chromatography.
 
9.1 What is adsorption?.
 
9.2 The eluotropic series.
 
9.3 Selectivity properties of the mobile phase.
 
9.4 Choice and optimization of the mobile phase.
 
9.5 Applications.
 
10 Reversed-Phase Chromatography.
 
10.1 Principle.
 
10.2 Mobile phases in reversed-phase chromatography.
 
10.3 Solvent selectivity and strength.
 
10.4 Stationary phases.
 
10.5 Method development in reversed-phase chromatography.
 
10.6 Applications

Report

"This book is very much ''an aid to this end and should be number one on every young chromatographer s book list. The publishers too have played their part in producing a book that is pleasing on the eye and a pleasure to read." (Chromatographia, 1 December 2010)

Product details

• Authors: Meyer, Veronica Meyer, Veronica R. Meyer, Veronika R. Meyer, Veronika R. (Empa St Gallen) Meyer, Vr Meyer, MEYER VERONICA R, Meyer Veronika R.
• Publisher: Wiley, John and Sons Ltd
• Languages: English
• Product format: Paperback / Softback
• Released: 09.04.2010
• EAN: 9780470682173
• ISBN: 978-0-470-68217-3
• No. of pages: 432
• Subjects: Natural sciences, medicine, IT, technology > Chemistry > Theoretical chemistry; Non-fiction book > Nature, technology > Natural science; Chemie, Analytische Chemie, Chromatographie, chemistry, Analytical Chemistry, Chromatographie / Trennverfahren, Chromatography / Separation Techniques