Thiol Redox Transitions in Cell Signaling, Part a - Chemistry and Biochemistry of Low Molecular Weight and Protein Thiols

Presents methods and protocols dealing with thiol oxidation-reduction reactions and their implications as they relate to cell signaling. This book deals with glutathionylation and dethiolation,and peroxide removal by peroxiredoxins/thioredoxins and glutathione peroxidases.

List of contents

1. His-tag switch method for the analysis of S-nitrosylated proteins


2. Identification of Protein Thiols in Mitochondrial Oxidative Phosphorylation Complexes


3. Mitochondrial thioredoxin reductase: purification, inhibitor studies, and role in cell signaling


4. assessing cell surface thiol status


5. Induction of Thioredoxin for Mediating Preconditioning-induced Cellular Responses


6. A trans sarcoplasmic reticulum membrane redox sensor in the striated muscle: exploring redox sensitivity of the ryanodine receptor calcium release channel


7. Rapid approach for the detection, quantification and discovery of novel sulphenic acid or S-nitrosothiol modified proteins using a biotin-switch method


8. Direct identification by mass spectrometry of in vivo S-nitrosylated peptides


9. Changing paradigms in theology: From antioxidant defence to redox regulation


10. Determination of GSNO formation in biological samples by HPLC electrochemical detection


11. Alteration of thioredoxin reductase 1 levels in elucidating cancer etiology


12. thiol-labeling technology in proteomics


13. Analytical methods for the determination of sulfur metabolite concentrations in cell extracts


14. Chemical tagging and mass spectrometry-based identification of protein thiols modified by lipid peroxidation-derived a,b-unsaturated aldehydes


15. Measuring protein thiol redox changes in mitochondria


16. Engineering of redox domains for monitoring electron transfers spectroscopically


17. Approaches to detection of cysteine sulfenic acids in proteins using dimedone-based chemical probes


18. Evaluation of conditions affecting degree of sulfenic acid labeling in redox-sensitive proteins


19. Colorimetric and spectrophotometric assays of sulfiredoxin


20. Quantifying disulfides in specific proteins


21. A simple method of detecting oxidatively-modified proteins


22. Role of Glutathione Conjugates in Cell Signaling


23. Regulation of Protein Function by Sulfinic Acid Formation


24. Mass spectrometry approaches for the study of the oxidation state of protein cysteine residues


25. assays of protein tyrosine phosphatase oxidation


26. oxidation state of cellular 2-Cys peroxiredoxins (reduced thiol, disulfide and hyperoxidised) by non-reducing SDS-PAGE and immunoblotting

Product details

• Authors: Enrique (EDT) Cadenas
• Assisted by: Enrique Cadenas (Editor), Lester Packer (Editor)
• Publisher: ELSEVIER SCIENCE BV
• Languages: English
• Product format: Hardback
• Released: 22.07.2010
• EAN: 9780123813459
• ISBN: 978-0-12-381345-9
• No. of pages: 416
• Series: Academic Press; Methods in Enzymology; Academic Press; Methods in Enzymology
• Subjects: Natural sciences, medicine, IT, technology > Biology > Biochemistry, biophysics; MEDICAL / Physiology, Cellular physiology