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Informationen zum Autor Dimitri Pappas is Assistant Professor in Analytical Chemistry at Texas Tech University. He obtained his B.S., University of Florida, 1998 followed by his Ph.D., University of Florida, 2002. He worked as a Research Scientist at Wyle Laboratories, NASA/Johnson Space Center, 2002-05 before taking up his post at Texas Tech. His key areas of research are: Analytical Fluorescence and Raman Spectroscopy; Cellular Analysis; Flow Cytometry; Optics With the focus on the interface between cell science and analytical chemistry. Dr. Pappas is currently developing novel analytical methods to study intact cells, cell digests, and tissue aggregates using spectroscopic and immunochemical techniques. The majority of his group's research is geared toward clinical analysis as well as medical testing in remote areas. Klappentext As analytical chemistry and biology move closer together, biologists are performing increasingly sophisticated analytical techniques on cells. Chemists are also turning to cells as a relevant and important sample to study newly developed methods. Practical Cell Analysis provides techniques, hints, and time-saving tips explaining what may be "common knowledge" to one field but are often hidden or unknown to another.Within this practical guide:* The procedures and protocols for cell separation, handling cells on a microscope and for using cells in microfluidic devices are presented.* Elements of cell culture are taken and combined with the practical advice necessary to maintain a cell lab and to handle cells properly during an analysis* The main chapters deal with the fundamentals and applied aspects of each technique, with one complete chapter focusing on statistical considerations of analyzing cells* Many diagram-based protocols for some of the more common cell processes are included* Chapter summaries and extensive tables are included so that key information can be looked up easily in the lab settingMuch like a good manual or cookbook this book is a useful, practical guide and a handy reference for all students, researchers and practitioners involved in cellular analysis. Zusammenfassung Practical Cell Analysis serves as a guide for researchers interested in adding cell analyses to their repertoire. This book covers the basics of setting up a cell culture-capable laboratory, as well as sources and types of cells. Inhaltsverzeichnis Chapter 1: Getting Starting (and Getting the Cells) 1.1 Introduction 1.2 The Driving Need 1.3 Primary and Cultured Cells 1.4 Choosing a Cultured Cell 1.5 Choosing Primary Cells 1.6 Easily Obtainable Primary Cells 1.7 Primary Cells from Tissues 1.8 Purifying Primary Cells 1.9 How Long do Primary Cells Remain Primary? 1.10 Obtaining Primary Cells from a Commercial Source 1.11 Bacteria and Yeast 1.12 Practical Aspects of Cell Culture 1.13 Safety Aspects of Primary and Transformed Cell Lines 1.14 Transfection of Primary and Transformed Cell Lines 1.15 Conclusion References Chapter 2: The Cell Culture Laboratory (Tools of the Trade) 2.1 Introduction 2.2 Issues Concerning a Cell Laboratory 2.3 Setting up a Cell Culture Laboratory 2.4 Cell Line Storage 2.5 Personal Protective Equipment 2.6 Cell and Sample Handling 2.7 Common Analytical Instrumentation for Cell Culture 2.8 Considerations when Setting up a Cell Culture Laboratory 2.9 Establishing and Regulating A Culture Facility 2.10 Conclusion References Chapter 3. Maintaining Cultures 3.1 Introduction 3.2 Medium 3.3 The Use of Medium in Analysis, and Alternatives 3.4 Culturing Cells Protocol 3.1: Sub-Culture of Adherent Cells 3.5 Growing Cells in Three Dimensions 3.6 Sterility and Contaminatio...
