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Informationen zum Autor H. BRIAN DUNFORD, PHD, is a biophysical chemist currently working on peroxidase enzymes and Professor Emeritus at the University of Alberta. He received his PhD from McGill University. He is the author of Heme Peroxidases , editor of other books, as well as coauthor of more than 250 journal articles. Klappentext Single source guide to peroxidases and catalasesReflecting the important historical discoveries and exciting research in the field in recent years, Peroxidases and Catalases: Biochemistry, Biophysics, Biotechnology and Physiology provides a much-needed systematic, up-to-date treatment of peroxidases and catalases.From the structure and properties of the various superfamilies to current applications of peroxidases, the book consolidates vast amounts of information previously scattered in the professional literature, covering all aspects of these ubiquitous enzymes that act on a variety of substances and processes in living systems-their properties, reactions, crystal structures, cloning, and more.Considering the subject from both theoretical and applied perspectives, Peroxidases and Catalases offers a critical review of the literature and detailed discussions of the most current research. Chapters cover:* The background and history of peroxidases and catalases* Plant, fungal, and bacterial peroxidase superfamilies and their organization* Mammalian peroxidases including medical and physiological roles* Spectroscopic and theoretical techniques for studying peroxidases highlighting the contributions of physicists and physical/theoretical chemists* Heme peroxidases, catalases, and other peroxidases such as vanadium and selenium peroxidase* Relevant plant and animal physiologyThis one-stop reference is a vital reference for biochemists, biologists, biochemical engineers, physiologists, environmental and pharmaceutical researchers, and others interested in the study and use of peroxidases and catalases. Zusammenfassung * Provides a summary of current research on peroxidase andcatalase enzymes which are ubiquitous in the plant and animalkingdoms. Inhaltsverzeichnis Preface xiii Contributors xv 1 Historical: Pioneering Work on Horseradish and Yeast Cytochrome c Peroxidases 1 Introduction 1 Techniques and Instrumentation 2 Summary and Conclusions 5 References 6 2 Heme Peroxidase and Catalase Families 9 Plant, Fungal, and Bacterial Peroxidases 9 Mammalian Peroxidases 11 Catalases 12 References 12 3 Horseradish Peroxidase. I. The Native Enzyme, Compounds I and II, Their Structures, and Their Cycle 13 Introduction 13 The Classic Peroxidase Cycle 13 Structure and Properties of Native Horseradish Peroxidase C 18 Horseradish Peroxidase Compound I (HRP-I) 23 Horseradish Peroxidase Compound II (HRP-II) 26 Some Diverse Approaches to an Understanding of Horseradish Peroxidase 27 References 30 4 Horseradish Peroxidase. II. Two-Electron Reactions, Ferrous Peroxidase, Compound III, The Five Oxidation States, Oxygen Evolution, and Inactivation 41 Introduction 41 Two-Electron Oxidations By Compound I 41 Oxygen Transfer By One-Electron Mechanisms 44 Ferrous Horseradish Peroxidase and Compound III 45 The Five Oxidation States of Horseradish Peroxidase 48 The Catalatic Reaction 50 The HRP Clock Reaction 51 Enzyme Inactivation 51 References 52 5 Horseradish Peroxidase. III. Oscillations and Peroxidase-Oxidase Reactions with NADH, Indole-3- Acetic Acid, and Isobutyraldehyde. Light Emission 57 Oscillations and the NADH Peroxidase-Oxidase Reaction 57 Peroxidase Oxidase Reaction with Indole-3-Acetic Acid 63 Reaction of Isobutyraldehyde with Horseradish Peroxidase 68 References 70
