Guide to Yeast Genetics: Functional Genomics, Proteomics and Other - Systems Analysi

Klappentext This fully updated edition of the best-selling three-part "Methods in Enzymology "series, "Guide to Yeast Genetics and Molecular Cell Biology "is specifically designed to meet the needs of graduate students, postdoctoral students, and researchers by providing all the up-to-date methods necessary to study genes in yeast. Procedures are included that enable newcomers to set up a yeast laboratory and to master basic manipulations. This volume serves as an essential reference for any beginning or experienced researcher in the field. Provides up-to-date methods necessary to study genes in yeast. Includes proceedures that enable newcomers to set up a yeast laboratory and to master basic manipulations. This volume serves as an essential reference for any beginning or experienced researcher in the field. Praise for the first edition: "...this is an excellent collection of methods that is certain to be an important handbook for yeast researchers."--Will Prinz, National Institutes of Health for ANALYTICAL BIOCHEMISTRY (April 2003) Zusammenfassung Part of the "Methods in Enzymology" series! this guide to yeast genetics and molecular cell biology is intends to meet the needs of graduate students! postdoctoral students! and researchers by providing the various methods necessary to study genes in yeast. It is suitable for beginning or experienced researchers in the field. Inhaltsverzeichnis PART I - Functional Genomics 1. Analysis of Gene Function using DNA Microarrays2. An introduction to microarray data analysis and visualization3. Genome-wide approaches to monitor pre-mRNA splicing4. ChIP-Seq: Using high-throughput DNA sequencing for genome-wide identification of transcription factor binding sites5. Genome-wide mapping of nucleosomes in yeast6. Genome-wide translational profiling by ribosome footprinting PART II - Systematic Genetic Analysis 7. Synthetic Genetic Array (SGA) Analysis in Saccharomyces cerevisiae and Schizosaccharomyces pombe8. Making temperature-sensitive mutants9. Quantitative Genetic Interaction Mapping using the E-MAP Approach10. Exploring gene function and drug action using chemogenomic dosage assays PART III - Proteomics 11. Yeast Expression Proteomics by High-Resolution Mass Spectrometry12. High-quality binary interactome mapping13. Quantitative analysis of protein phosphorylation on a system-wide scale by mass spectrometry based proteomics14. A Toolkit of Protein-fragment Complementation Assays for Studying and Dissecting Large-scale and Dynamic Protein-Protein Interactions in Living Cells15. Yeast Lipid Analysis and Quantification by Mass Spectrometry16. Mass Spectrometry-Based Metabolomics of Yeast PART IV - Systems Analysis 17. Imaging single mRNA molecules in yeast18. Reconstructing gene histories in Ascomycota fungi19. Experimental evolution in yeast: a practical guide PART V - Synthetic Biology 20. Enhancing stress resistance and production phenotypes through transcriptome engineering PART VI - Advances in Cytology/Biochemistry 21. Visualizing yeast chromosomes and nuclear architecture22. Quantitative localization of chromosomal loci by immunofluorescence23. Spinning Disk Confocal Microscopy of Yeast24. Correlative GFP-immunoelectron microscopy in yeast25. Analyzing P-bodies and stress granules in Saccharomyces cerevisiae26. Analyzing mRNA expression using single mRNA resolution fluorescent in situ hybridization27. The Use of In Vitro Assays to Measure Endoplasmic Reticulum Associated Degradation (ERAD)28. A protein transformation protocol for introducing yeast prion particles into yeast29. Overexpression and Purification of Integral Membrane Proteins in Yeast30. Biochemical, cell biological and genetic assays to analyze amyloid and prion aggregation in yeast PART VII - Other Fungi 31. Genetics and Molecular Biology in Candida albicans32. Molecular Genetics of Schizosaccharomyces pombe33. Applyi...

List of contents

PART I - Functional Genomics1. Analysis of Gene Function using DNA Microarrays2. An introduction to microarray data analysis and visualization3. Genome-wide approaches to monitor pre-mRNA splicing4. ChIP-Seq: Using high-throughput DNA sequencing for genome-wide identification of transcription factor binding sites5. Genome-wide mapping of nucleosomes in yeast6. Genome-wide translational profiling by ribosome footprintingPART II - Systematic Genetic Analysis7. Synthetic Genetic Array (SGA) Analysis in Saccharomyces cerevisiae and Schizosaccharomyces pombe8. Making temperature-sensitive mutants9. Quantitative Genetic Interaction Mapping using the E-MAP Approach10. Exploring gene function and drug action using chemogenomic dosage assaysPART III - Proteomics11. Yeast Expression Proteomics by High-Resolution Mass Spectrometry12. High-quality binary interactome mapping13. Quantitative analysis of protein phosphorylation on a system-wide scale by mass spectrometry based proteomics14. A Toolkit of Protein-fragment Complementation Assays for Studying and Dissecting Large-scale and Dynamic Protein-Protein Interactions in Living Cells15. Yeast Lipid Analysis and Quantification by Mass Spectrometry16. Mass Spectrometry-Based Metabolomics of YeastPART IV - Systems Analysis17. Imaging single mRNA molecules in yeast18. Reconstructing gene histories in Ascomycota fungi19. Experimental evolution in yeast: a practical guidePART V - Synthetic Biology20. Enhancing stress resistance and production phenotypes through transcriptome engineeringPART VI - Advances in Cytology/Biochemistry21. Visualizing yeast chromosomes and nuclear architecture22. Quantitative localization of chromosomal loci by immunofluorescence23. Spinning Disk Confocal Microscopy of Yeast24. Correlative GFP-immunoelectron microscopy in yeast25. Analyzing P-bodies and stress granules in Saccharomyces cerevisiae26. Analyzing mRNA expression using single mRNA resolution fluorescent in situ hybridization27. The Use of In Vitro Assays to Measure Endoplasmic Reticulum Associated Degradation (ERAD)28. A protein transformation protocol for introducing yeast prion particles into yeast29. Overexpression and Purification of Integral Membrane Proteins in Yeast30. Biochemical, cell biological and genetic assays to analyze amyloid and prion aggregation in yeastPART VII - Other Fungi31. Genetics and Molecular Biology in Candida albicans32. Molecular Genetics of Schizosaccharomyces pombe33. Applying Genetics and Molecular Biology to the Study of the Human Pathogen Cryptococcus neoformans34. The Fungal Genome Initiative and lessons learned from genome sequencing35. Ultradian metabolic cycles in yeast

Report

Praise for the first edition:"...this is an excellent collection of methods that is certain to be an important handbook for yeast researchers." --Will Prinz, National Institutes of Health for ANALYTICAL BIOCHEMISTRY (April 2003)

Product details

• Authors: Gerald R. Fink, Christine Guthrie, Jonathan Weissman
• Assisted by: Gerald R. Fink (Editor), Christine Guthrie (Editor), Jonathan Weissman (Editor)
• Publisher: ELSEVIER SCIENCE BV
• Languages: English
• Product format: Hardback
• Release: 20.06.2011, delayed
• EAN: 9780123751720
• ISBN: 978-0-12-375172-0
• No. of pages: 942
• Series: Methods in Enzymology; Methods in Enzymology
• Subjects: Natural sciences, medicine, IT, technology > Biology > General, dictionaries; SCIENCE / Life Sciences / Molecular Biology, SCIENCE / Life Sciences / Cell Biology, SCIENCE / Life Sciences / Mycology, molecular biology, Cellular biology (cytology), Mycology, fungi (non-medical), Mycology, fungi