Guide to Yeast Genetics: Functional Genomics, Proteomics and Other - Systems Analysi

Klappentext This fully updated edition of the best-selling three-part "Methods in Enzymology "series, "Guide to Yeast Genetics and Molecular Cell Biology "is specifically designed to meet the needs of graduate students, postdoctoral students, and researchers by providing all the up-to-date methods necessary to study genes in yeast. Procedures are included that enable newcomers to set up a yeast laboratory and to master basic manipulations. Relevant background and reference information given for procedures can be used as a guide to developing protocols in a number of disciplines. This volume serves as an essential reference for any beginning or experienced researcher in the field. Provides up-to-date methods necessary to study genes in yeast. Includes proceedures that enable newcomers to set up a yeast laboratory and to master basic manipulations. This volume serves as an essential reference for any beginning or experienced researcher in the field. Praise for the first edition: "...this is an excellent collection of methods that is certain to be an important handbook for yeast researchers."--Will Prinz, National Institutes of Health for ANALYTICAL BIOCHEMISTRY (April 2003) Zusammenfassung Part of the "Methods in Enzymology" series! this guide to yeast genetics and molecular cell biology intends to meet the needs of graduate students! postdoctoral students! and researchers by providing the various methods necessary to study genes in yeast. It is suitable for beginning or experienced researchers in the field. Inhaltsverzeichnis PART I - Functional Genomics 1.Analysis of Gene Function using DNA Microarrays Andrew P. Capaldi 2.An introduction to microarray data analysis and visualization Gregg B. Whitworth 3. Genome-wide approaches to monitor pre-mRNA splicing Maki Inada and Jeff Pleiss 4. ChIP-Seq: Using high-throughput DNA sequencing for genome-wide identification of transcription factor binding sites Philippe Lefrançois, Wei Zheng and Michael Snyder 5. Genome-wide mapping of nucleosomes in yeast Oliver J. Rando 6. Genome-wide translational profiling by ribosome footprinting Nicholas T. Ingolia PART II - Systematic Genetic Analysis 7. Synthetic Genetic Array (SGA) Analysis in Saccharomyces cerevisiae and Schizosaccharomyces pombe Anastasia Baryshnikova, Michael Costanzo, Scott Dixon, Franco J. Vizeacoumar, Chad L. Myers, Brenda Andrews and Charles Boone 8. Making temperature-sensitive mutants Shay Ben-Aroya, Xuewen Pan, Jef D. Boeke and Philip Hieter 9. Quantitative Genetic Interaction Mapping using the E-MAP Approach Sean R. Collins, Assen Roguev and Nevan J. Krogan 10. Exploring gene function and drug action using chemogenomic dosage assays Elke Ericson, Shawn Hoon, Robert P. St. Onge, Guri Giaever and Corey Nislow PART III - Proteomics 11. Yeast Expression Proteomics by High-Resolution Mass Spectrometry Tobias C. Walther, Jesper V. Olsen and Matthias Mann 12. High-quality binary interactome mapping Matija Dreze, Dario Monachello, Claire Lurin, Michael E. Cusick, David E. Hill, March Vidal, and Pascal Braun 13. Quantitative analysis of protein phosphorylation on a system-wide scale by mass spectrometry based proteomics Bernd Bodenmiller and Rudi Aebersold 14. A Toolkit of Protein-fragment Complementation Assays for Studying and Dissecting Large-scale and Dynamic Protein-Protein Interactions in Living Cells Stephen W. Michnick, Po-Hien Ear, Christian Landry, Mohan K. Malleshaiah and Vincent Messier 15. Yeast Lipid Analysis and Quantification by Mass Spectrometry Xue Li Guan, Isabelle Riezman, Markus R. Wenk and Howard Riezman 16. Mass Spectrometry-Based Metabolomics of Yeast Christopher A. Crutchfield, Wenyun Lu, Eugene Melamu...

List of contents

PART I - Functional Genomics
1.Analysis of Gene Function using DNA Microarrays
Andrew P. Capaldi
2.An introduction to microarray data analysis and visualization
Gregg B. Whitworth
3. Genome-wide approaches to monitor pre-mRNA splicing
Maki Inada and Jeff Pleiss
4. ChIP-Seq: Using high-throughput DNA sequencing for genome-wide identification of transcription factor binding sites
Philippe Lefrançois, Wei Zheng and Michael Snyder
5. Genome-wide mapping of nucleosomes in yeast
Oliver J. Rando
6. Genome-wide translational profiling by ribosome footprinting
Nicholas T. Ingolia

PART II - Systematic Genetic Analysis
7. Synthetic Genetic Array (SGA) Analysis in Saccharomyces cerevisiae and Schizosaccharomyces pombe
Anastasia Baryshnikova, Michael Costanzo, Scott Dixon, Franco J. Vizeacoumar, Chad L. Myers, Brenda Andrews and Charles Boone
8. Making temperature-sensitive mutants
Shay Ben-Aroya, Xuewen Pan, Jef D. Boeke and Philip Hieter
9. Quantitative Genetic Interaction Mapping using the E-MAP Approach
Sean R. Collins, Assen Roguev and Nevan J. Krogan
10. Exploring gene function and drug action using chemogenomic dosage assays
Elke Ericson, Shawn Hoon, Robert P. St. Onge, Guri Giaever and Corey Nislow

PART III - Proteomics
11. Yeast Expression Proteomics by High-Resolution Mass Spectrometry
Tobias C. Walther, Jesper V. Olsen and Matthias Mann
12. High-quality binary interactome mapping
Matija Dreze, Dario Monachello, Claire Lurin, Michael E. Cusick, David E. Hill, March Vidal, and Pascal Braun
13. Quantitative analysis of protein phosphorylation on a system-wide scale by mass spectrometry based proteomics
Bernd Bodenmiller and Rudi Aebersold
14. A Toolkit of Protein-fragment Complementation Assays for Studying and Dissecting Large-scale and Dynamic Protein-Protein Interactions in Living Cells
Stephen W. Michnick, Po-Hien Ear, Christian Landry, Mohan K. Malleshaiah and Vincent Messier
15. Yeast Lipid Analysis and Quantification by Mass Spectrometry
Xue Li Guan, Isabelle Riezman, Markus R. Wenk and Howard Riezman
16. Mass Spectrometry-Based Metabolomics of Yeast
Christopher A. Crutchfield, Wenyun Lu, Eugene Melamud, and Joshua D. Rabinowitz

PART IV - Systems Analysis
17. Imaging single mRNA molecules in yeast
Hyun Youk, Arjun Raj and Alexander van Oudenaarden
18. Reconstructing gene histories in Ascomycota fungi
IlanWapinski and Aviv Regev
19. Experimental evolution in yeast: a practical guide
Maitreya J. Dunham

PART V - Synthetic Biology
20. Enhancing stress resistance and production phenotypes through transcriptome engineering
Felix H. Lam, Franz S. Hartner, Gerald R. Fink, and Gregory Stephanopoulos

PART VI - Advances in Cytology/Biochemistry
21. Visualizing yeast chromosomes and nuclear architecture
Peter Meister, Lutz R. Gehlen, Elisa Varela, Veronique Kalck and Susan M. Gasser
22. Quantitative localization of chromosomal loci by immunofluorescence
Donna Garvey Brickner, William Light and Jason H. Brickner
23. Spinning Disk Confocal Microscopy of Yeast
Kurt Thorn
24. Correlative GFP-immunoelectron microscopy in yeast
Christopher Buser and Kent McDonald
25. Analyzing P-bodies and stress granules in Saccharomyces cerevisiae
J Ross Buchan, Tracy Nissan and Roy Parker
26. Analyzing mRNA expression using single mRNA resolution fluorescent in situ hybridization
Daniel Zenklusen and Robert H Singer
27. The Use of In Vitro Assays to Measure Endoplasmic Reticulum Associated Degradation (ERAD)
Jeffrey L. Brodsky
28. A protein transformation protocol for introducing yeast prion particles into yeast
Motomasa Tanaka

Report

Praise for the first edition:
"...this is an excellent collection of methods that is certain to be an important handbook for yeast researchers."
-- Will Prinz, National Institutes of Health for ANALYTICAL BIOCHEMISTRY, April 2003

Product details

• Authors: Gerald R. Fink, Christine Guthrie, Jonathan Weissman
• Assisted by: Gerald R. Fink (Editor), Christine Guthrie (Editor), Jonathan Weissman (Editor)
• Publisher: ELSEVIER SCIENCE BV
• Languages: English
• Product format: Paperback / Softback
• Released: 20.06.2011
• EAN: 9780123751713
• ISBN: 978-0-12-375171-3
• No. of pages: 944
• Series: Methods in Enzymology; Methods in Enzymology
• Subject: Natural sciences, medicine, IT, technology > Biology > Botany