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Klappentext Every cell of the body is dependent on calcium to function. Calcium is found in teeth and bones, and calcium signalling is necessary for the movement of muscles and for the action of the heart and the intestines as well as blood coagulation. Calcium in Living Cells will update classic techniques in detecting microscopic levels of calcium ions (Ca2+) in living cells, as well as address new techniques in the field of calcium detection and calcium signaling. Such detection and measurement of intracellular calcium is important to researchers studying the heart, musculoskeletal, gastrointestinal, and immune systems, whose findings will aid in the advancement of drug and genomic therapies to treat heart, gastrointestinal, autoimmune, and infectious diseases. Zusammenfassung Covers classic techniques in detecting microscopic levels of calcium ions (Ca2+) in living cells! as well as addresses techniques in the field of calcium detection and calcium signaling. This title gives researchers much needed information on how to study calcium in live cells. Inhaltsverzeichnis A Practical Guide to the Preparation of Ca2+ Buffers Donald M. Bers, Chris W. Patton and Richard Nuccitelli Photorelease Techniques for Raising or Lowering Intracellular Ca2+ Robert Zucker Making and Using Calcium-Selective Mini- and Microelectrodes L. Hove-Madsen, S. Baudet and D.M. Bers Construction, Theory and Practical Considerations for using Self-Referencing of Ca2+-Selective Microelectrodes for Monitoring Extracellular Ca2+ Gradients Mark A. Messerli and Peter J.S. Smith Practical Aspects of Measuring Intracellular Calcium Signals with Fluorescent Indicators Joseph P. Y. Kao, Gong Li, Darryl A. Auston Genetically-encoded probes for measurement of intracellular calcium Michael Whitaker Patch clamp methods for studying calcium channels David L. Armstrong, Christian Erxleben and Jody A. White Nuclear Patch-Clamp Recording from Inositol 1,4,5-Trisphosphate Receptors Taufiq Rahman, Colin W. Taylor Confocal And Multiphoton Imaging Of Intracellular Ca2+ Godfrey Smith, Martyn Reynolds, Francis Burto1, Ole Johan Kemi The Use of Aequorins to Record and Visualize Ca2+ Dynamics: From Subcellular Microdomains to Whole Organisms. Sarah E. Webb, Kelly L. Rogers, Eric Karplus, and Andrew L. Miller ...
