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Tying together technological and conceptual advances of the past decade, this seminal book introduces the novel approach of chemical cytometry -- the biochemical analysis of cell constituents at the single cell level. As such, it combines recently developed methods for the handling of single cells, the separation of their components, and the detection and analysis of biomolecular species with application examples from cell biology, neurobiology and molecular medicine.The editor has succeeded in acquiring contributions from the recognized leaders in the field, resulting in a complete and authoritative overview for both developers as well as users of these new methods.
List of contents
PrefaceORIGIN, CURRENT STATUS, AND FUTURE PERSPECTIVES OF CHEMICAL CYTOMETRYThe Cell and CytometryFlow and Image CytometryChemical CytometryChemical Cytometry of DNA and mRNAMetabolic CytometryFuture Perspectives on Instrumentation for Chemical CytometryMETABOLIC CYTOMETRY - THE STUDY OF GLYCOSPHINGOLIPID METABOLISM IN SINGLE PRIMARY CELLS OF THE DORSAL ROOT GANGLIAIntroductionMaterial and MethodsResults and DiscussionConclusionsCELL SIGNALING STUDIES AT THE SINGLE-CELL LEVELIntroductionAnalytes Examined and Reporters UsedCell Preparation and Reporter LoadingCell Lysis and Sampling TechniquesElectrophoresis Separation ConditionsDetectionAutomation and ThroughputULTRASENSITIVE DETECTION OF LOW-COPY-NUMBER MOLECULES FROM SINGLE CELLSIntroductionMicrochip Designs for Single-Cell Analysis and/or Cell ManipulationUltrasensitive Detection Methods for Single-Cell AnalysisSingle-Cell Analysis with Single-Molecule Sensitivity on Integrated Microfluidic ChipConclusionsCAPILLARY ELECTROPHORESIS OF NUCLEIC ACIDS AT THE SINGLE-CELL LEVELIntroductionOn-Line Cell AnalysisDirect Gene and Gene Expression Analysis Without AmplificationPotential Alternative Techniques for Single-Cell Gene and Gene Expression AnalysisConclusionsMICROFLUIDIC TECHNOLOGY FOR SINGLE-CELL ANALYSISIntroductionBiological Significance of Single-Cell AnalysisMicrofluidic Devices in Our LaboratoriesMaterials, Methods, and ProtocolsConclusionsON-CHIP ELECTROPORATION AND ELECTROFUSION FOR SINGLE-CELL ENGINEERINGIntroductionSingle-Cell Electroporation in Microfluidic DevicesSingle-Cell Electrofusion in Microfluidic DevicesConclusionsELECTROPORATIVE FLOW CYTOMETRY FOR SINGLE-CELL ANALYSISIntroductionFlow-Through Electroporation under Constant VoltageElectroporative Flow Cytometry for Detecting Protein TranslocationElectroporative Flow Cytometry for Measuring Single-Cell BiomechanicsElectroporative Flow Cytometry for Selectively Releasing and Analyzing Specific Intracellular MoleculesConclusionULTRASENSITIVE ANALYSIS OF INDIVIDUAL CELLS VIA DROPLET MICROFLUIDICSIntroductionDroplet PropertiesDroplet GenerationCell EncapsulationDroplet ManipulationDroplet Concentration ControlTemperature Control of DropletsDetection in DropletsConclusionsPROBING EXOCYTOSIS AT SINGLE CELLS USING ELECTROCHEMISTRYIntroductionMeasurement RequirementsElectrode FabricationMeasurements at Single CellsFusion Pore DynamicsConclusionsELECTROCHEMICAL DETERMINATION OF ENZYME ACTIVITY IN SINGLE CELLSIntroductionElectrochemical Detection Coupled with Capillary ElectrophoresisVoltammetryScanning Electrochemical Microscopy (SECM)High-Throughput ECDPerspectiveSINGLE-CELL MASS SPECTROMETRYIntroductionMass SpectrometryOverall Outlook for Single-Cell MSOPTICAL SENSING ARRAYS FOR SINGLE-CELL ANALYSISIntroduction to Fiber-Optic Single-Cell ArraysAdvantages of Fiber-Optic Single-Cell ArraysFiber-Optic ArraysSinge-Cell Arrays for BacteriaSingle-Cell Arrays for YeastSingle-Cell Arrays for Mammalian CellsImage and Data Analysis for Single-Cell ArraysSummary
About the author
Chang Lu is a professor of biological engineering at Purdue University, West Lafayette (USA). Having obtained his academic degrees from Peking University and from the University of Illinois at Urbana-Champaign, he received post-doctoral training at Cornell University before moving to Purdue. His current research is focused on the application of microfluidics and spectroscopy tools to study single cells. He has received a number of awards, including the NSF career award and the Wallace H. Coulter foundation Early Career Award in Biomedical Engineering.
Summary
Tying together technological and conceptual advances of the past decade, this seminal book introduces the novel approach of chemical cytometry -- the biochemical analysis of cell constituents at the single cell level. As such, it combines recently developed methods for the handling of single cells, the separation of their components, and the detection and analysis of biomolecular species with application examples from cell biology, neurobiology and molecular medicine.
The editor has succeeded in acquiring contributions from the recognized leaders in the field, resulting in a complete and authoritative overview for both developers as well as users of these new methods.
