Functional Glycomics - Methods and Protocols

The study of functional glycomics requires the continuous development of rapid and sensitive methods for the identification of glycan structures and integration to structure-function relationships. In Functional Glycomics: Methods and Protocols, a panel of world-renowned experts provide new developments and emerging glycomics techniques in the form of detailed protocols exploring the fundamental challenges and most cutting-edge novel applications. Written in the highly successful Methods in Molecular Biology(TM) series format, chapters in this volume present brief introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes sections, highlighting key tips on troubleshooting and avoiding known pitfalls.
Authoritative and easy to use, Functional Glycomics: Methods and Protocols serves as an ideal reference for scientists working in biochemistry, molecular biology, cell biology, immunology, microbiology, and virology and a guide to appropriate techniques and the design of achievable research plans in this vital field.

List of contents

Functional Glycomics and Glycobiology: An Overview.- Historical Overview of Glycoanalysis.- Quantitative Glycomics.- Human Gangliosides and Bacterial Lipo-oligosaccharides in the Development of Autoimmune Neuropathies.- Biotinylated Multivalent Glycoconjugates for Surface Coating.- Profiling LPS Glycoforms of Non-typeable Haemophilus influenzae by Multiple-Stage Tandem Mass Spectrometry.- In Vitro Reconstitution of Escherichia coli O86 O Antigen Repeating Unit.- Glycoprotein Characterization.- N-Linked Glycoprotein Analysis Using Dual-Extraction Ultrahigh-Performance Liquid Chromatography and Electrospray Tandem Mass Spectrometry.- Microarray-Based Study of Carbohydrate-Protein Binding.- The Application of NMR Spectroscopy to Functional Glycomics.- Metabolomics in Glycomics.- Characterization of Lipid-Linked Oligosaccharides by Mass Spectrometry.- A Glycomics Approach to the Discovery of Potential Cancer Biomarkers.- Extraction of Chondroitin/Dermatan Sulfate Glycosaminoglycans from Connective Tissue for Mass Spectrometric Analysis.- N-Linked Protein Glycosylation in a Bacterial System.- Characterization of Polysaccharides Using Mass Spectrometry for Bacterial Serotyping.- Is Permethylation Strategy Always Applicable to Protein N-Glycosylation Study?: A Case Study on the O-Acetylation of Sialic Acid in Fish Serum Glycans.- Bioinformatics in Glycomics: Glycan Characterization with Mass Spectrometric Data Using SimGlycan(TM).

Summary

The study of functional glycomics requires the continuous development of rapid and sensitive methods for the identification of glycan structures and integration to structure-function relationships. In Functional Glycomics: Methods and Protocols, a panel of world-renowned experts provide new developments and emerging glycomics techniques in the form of detailed protocols exploring the fundamental challenges and most cutting-edge novel applications. Written in the highly successful Methods in Molecular Biology™ series format, chapters in this volume present brief introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes sections, highlighting key tips on troubleshooting and avoiding known pitfalls.
Authoritative and easy to use, Functional Glycomics: Methods and Protocols serves as an ideal reference for scientists working in biochemistry, molecular biology, cell biology, immunology, microbiology, and virology and a guide to appropriate techniques and the design of achievable research plans in this vital field.

Additional text

From the reviews:
“This book provides a useful introduction to the field of glycobiology as well as detailed protocols relating to methods of analyses. … relevance to the microbiologist who is considering exploring this field and wishes to gain an understanding of current methodologies.” (Sheila Patrick, Microbiology Today, 2010)
“The book consists of 19 chapters prepared by an international team of authors. … book will be very useful source for many researchers in glycobiology fields. It should be recommended also to students and teachers of colleges and universities who specialized in glycobiology. I would like to mention that the book is well done including a materials and methods part to each chapter, references, subject index, figures and tables.” (G. Ya. Wiederschain, Biochemistry, Vol. 75 (11), 2010)

Report

From the reviews:
"This book provides a useful introduction to the field of glycobiology as well as detailed protocols relating to methods of analyses. ... relevance to the microbiologist who is considering exploring this field and wishes to gain an understanding of current methodologies." (Sheila Patrick, Microbiology Today, 2010)
"The book consists of 19 chapters prepared by an international team of authors. ... book will be very useful source for many researchers in glycobiology fields. It should be recommended also to students and teachers of colleges and universities who specialized in glycobiology. I would like to mention that the book is well done including a materials and methods part to each chapter, references, subject index, figures and tables." (G. Ya. Wiederschain, Biochemistry, Vol. 75 (11), 2010)

Product details

• Assisted by: Jianju li (Editor), Jianjun Li (Editor)
• Publisher: Springer, Berlin
• Languages: English
• Product format: Hardback
• Released: 31.12.2011
• EAN: 9781607614531
• ISBN: 978-1-60761-453-1
• No. of pages: 284
• Dimensions: 189 mm x 25 mm x 258 mm
• Weight: 730 g
• Illustrations: XI, 284 p.
• Series: Methods in Molecular Biology; Methods in Molecular Biology
• Subject: Natural sciences, medicine, IT, technology > Biology > Biochemistry, biophysics