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Due to its prolific reproduction and the external development of the transparent embryo, the zebrafish is the prime model for genetic and developmental studies, as well as research in genomics. Part of the "Reliable Lab Solutions" series dealing with zebrafish, this title brings together a collection of time-tested methods.
List of contents
1. Haploid Screens and Gamma-Ray Mutagenesis (Charline Walker) 2. Positional Cloning of Mutated Zebrafish Genes (Will Talbot) 3. The Zebrafish Genome (John Postlethwait) 4. Retroviral-Mediated Insertional Mutogenesis in Zebrafish (Adam Amsterdam) 5. Genetic Screens for Maternal-Effect Mutations (Francisco Pelegri) 6. Behavior Screening Assays in Zebrafish (Herwig Baier) 7. A High-Throughput Method for Identifying N-Ethyl-N-Nitrosourea (ENU)-Induced Point Mutations in Zebrafish (Cecelia B. Moens) 8. Transgenesis and Gene Trap Methods in Zebrafish by Using the Tol2 Transposable Element (Koichi Kawakami)9. Molecular Cytogenetic Methodologies and a Bacterial Artificial Chromosome (BAC) Probe Panel Resource for Genomic Analysis in Zebrafish (Charles Lee) 10. The Zon Laboratory Guide to Positional Cloning in Zebrafish (Leonard I. Zon) 11. Sleeping Beauty Transposon for Efficient Gene Delivery (Stephen C. Ecker) 12. Transgene Manipulation in Zebrafish by Using Recombinases (Gary W. Stuart) 13. Highly Efficient Zebrafish Transgenesis Mediated by the Meganuclease I-SceI (Joachim Wittbrodt) 14. Cloning Zebrafish by Nuclear Transfer (Shuo Lin) 15. Spatial and Temporal Expression of the Zebrafish Genome by Large-Scale In Situ Hybridization Screening (Christine Thisse) 16. Genetic Backgrounds, Standard Lines, and Husbandry of Zebrafish (Bill Trevarrow) 17. Common Diseases of Laboratory Zebrafish (Jennifer L. Matthews) 18. Zebrafish Sperm Cryopreservation (Thomas Look)
