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Klappentext This volume, as part of the Reliable Lab Solution Series, delivers such a tool offering busy researchers across many disciplines a handy resource of all the best methods and protocols for Cytometry to use at the bench. Zusammenfassung Highlights some of the top downloaded and cited chapters! authored by pioneers in the field and enhanced with their tips! and pitfalls to avoid. This book includes protocols developed and used by leaders in the field! and refines! organizes and updates popular methods. Inhaltsverzeichnis Part I: Fluorochromes/General Techniques 1. Principles of confocal microscopy (Robinson) 2. Protein labeling with fluorescent probes (Holmes, Lantz) 3. Cytometry of fluorescence resonance energy transfer (Vereb, Matko, Szollosi) 4. The rainbow of fluorescent proteins (Galbraith) 5. Labeling cellular targets with semiconductor quantum dot conjugates (Wu, Bruchez) 6. High-gradient magnetic cell sorting (Radbruch, Mechtold, Thiel, Miltenyi, Pfluger) 7. Multiplexed microsphere assays for protein and DNA binding reactions (Kellar, Oliver) 8. Biohazard sorting (Schmid, Roederer, Koup, Ambrozak, Perfetto) 9. Guidelines for the presentation of flow cytometric data. (Roederer, Darzynkiewicz, Paks) Part II: Cellular DNA Content Analysis 10. VinDetergent and proteolytic enzyme-based techniques for nuclear isolation and DNA content analysis (Vindelov,Christensen) 11. Rapid DNA content analysis (Krishan) 12. DNA analysis from paraffin-embedded blocks (Hedley) 13. Flow cytometry and sorting of plant protoplasts and cells (Galbraith) 14. DNA content histogram and cell cycle analysis. (Rabinovitch) 15. Simultaneous analysis of cellular RNA and DNA content (Darzynkiewicz) Part III. Cell Proliferation and Death Assays 16. Immunochemical quantitation of bromodeoxyuridine: application to cell cycle kinetics (Dolbeare, Selden)...
List of contents
Part I: Fluorochromes/General Techniques
1. Principles of confocal microscopy
(Robinson)
2. Protein labeling with fluorescent probes
(Holmes, Lantz)
3. Cytometry of fluorescence resonance energy transfer
(Vereb, Matko, Szollosi)
4. The rainbow of fluorescent proteins
(Galbraith)
5. Labeling cellular targets with semiconductor quantum dot conjugates
(Wu, Bruchez)
6. High-gradient magnetic cell sorting
(Radbruch, Mechtold, Thiel, Miltenyi, Pfluger)
7. Multiplexed microsphere assays for protein and DNA binding reactions
(Kellar, Oliver)
8. Biohazard sorting
(Schmid, Roederer, Koup, Ambrozak, Perfetto)
9. Guidelines for the presentation of flow cytometric data.
(Roederer, Darzynkiewicz, Paks)
Part II: Cellular DNA Content Analysis
10. VinDetergent and proteolytic enzyme-based techniques for nuclear isolation and DNA content analysis
(Vindelov,Christensen)
11. Rapid DNA content analysis
(Krishan)
12. DNA analysis from paraffin-embedded blocks
(Hedley)
13. Flow cytometry and sorting of plant protoplasts and cells
(Galbraith)
14. DNA content histogram and cell cycle analysis.
(Rabinovitch)
15. Simultaneous analysis of cellular RNA and DNA content
(Darzynkiewicz)
Part III. Cell Proliferation and Death Assays
16. Immunochemical quantitation of bromodeoxyuridine: application to cell cycle kinetics
(Dolbeare, Selden)
