Type 2 Diabetes - Methods and Protocols

Diabetes is now reaching epidemic proportions, and the associated complications of this disease can be disabling and even life-threatening. In Type 2 Diabetes: Methods and Protocols, leading investigators provide up-to-date explanations of commonly used laboratory protocols used in diabetes research. Covering the commonly described in vivo and in vitro model systems, the volume ultimately leads to an overall view of how cellular dysfunction and degeneration leads to susceptibility and diabetes disease progression. Written in the highly successful Methods in Molecular Biology(TM) series format, chapters include brief introductions to their respective subjects, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and expert notes on troubleshooting and avoiding known pitfalls.
Comprehensive and cutting-edge, Type 2 Diabetes: Methods and Protocols offers succinct, proven techniques to aid research scientists and clinicians in continuing the study of this debilitating disease.

List of contents

Selecting the "Right" Mouse Model for Metabolic Syndrome and Type 2 Diabetes Research.- Nutritional Models of Type 2 Diabetes Mellitus.- The Isolation and Purification of Rodent Pancreatic Islets of Langerhans.- The Measurement of Insulin Secretion from Isolated Rodent Islets of Langerhans.- The Incubation and Monitoring of Cell Viability in Primary Rat Islets of Langerhans and Pancreatic ?-Cell Lines.- In Vitro Culture of Isolated Islets of Langerhans: Analysis of Function.- Single-Cell RT-PCR Identification of Genes Expressed by Human Islet Endocrine Cells.- Laser Capture Microdissection of Human Pancreatic ?-Cells and RNA Preparation for Gene Expression Profiling.- In Vitro Transdifferentiation of Human Hepatoma Cells into Pancreatic-Like Cells.- The Measurement of GLUT4 Translocation in 3T3-L1 Adipocytes.- Pancreatic Remodeling: Beta-Cell Apoptosis, Proliferation and Neogenesis, and the Measurement of Beta-Cell Mass and of Individual Beta-Cell Size.- Morphology of Pancreatic Islets: A Time Course of Pre-diabetes in Zucker Fatty Rats.- Fluorescent Immunohistochemistry and In Situ Hybridization Analysis of Pancreas.- The Measurement of Insulin Secretion Using Pancreas Perfusion in the Rodent.- Hyperinsulinemic-Euglycemic Clamp to Assess Insulin Sensitivity In Vivo.- Gene Expression Analysis in Diabetes Research.- Gene Expression Mining in Type 2 Diabetes Research.

Summary

Diabetes is now reaching epidemic proportions, and the associated complications of this disease can be disabling and even life-threatening. In Type 2 Diabetes: Methods and Protocols, leading investigators provide up-to-date explanations of commonly used laboratory protocols used in diabetes research. Covering the commonly described in vivo and in vitro model systems, the volume ultimately leads to an overall view of how cellular dysfunction and degeneration leads to susceptibility and diabetes disease progression. Written in the highly successful Methods in Molecular Biology™ series format, chapters include brief introductions to their respective subjects, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and expert notes on troubleshooting and avoiding known pitfalls.

Comprehensive and cutting-edge, Type 2 Diabetes: Methods and Protocols offers succinct, proven techniques to aid research scientists and clinicians in continuing the study of this debilitating disease.

Additional text

From the reviews:
“This book provides in 17 chapters laboratory protocols used in diabetes research. It describes methods using new technologies in studying pancreatic beta cells. For research diabetologists.” (Pediatric Endocrinology Reviews, Vol. 7 (4), June, 2010)
“Written for those engaged in basic science research who have an interest in expanding their repertoire of techniques for the study of diabetes. … a credible source of information for those seeking experimental advice. … an excellent compilation of techniques for the laboratory study of diabetes, particularly those who are interested in studying pancreatic beta cells. Many techniques are covered, with up-to-date and relevant references as well as step-by-step instructions for performing them. The book is a useful addition to any diabetes researcher’s bookshelf.” (Robert Michael Sargis, Doody’s Review Service, July, 2010)

Report

From the reviews:
"This book provides in 17 chapters laboratory protocols used in diabetes research. It describes methods using new technologies in studying pancreatic beta cells. For research diabetologists." (Pediatric Endocrinology Reviews, Vol. 7 (4), June, 2010)
"Written for those engaged in basic science research who have an interest in expanding their repertoire of techniques for the study of diabetes. ... a credible source of information for those seeking experimental advice. ... an excellent compilation of techniques for the laboratory study of diabetes, particularly those who are interested in studying pancreatic beta cells. Many techniques are covered, with up-to-date and relevant references as well as step-by-step instructions for performing them. The book is a useful addition to any diabetes researcher's bookshelf." (Robert Michael Sargis, Doody's Review Service, July, 2010)

Product details

• Assisted by: Clair Stocker (Editor), Claire Stocker (Editor)
• Publisher: Springer, Berlin
• Languages: English
• Product format: Hardback
• Released: 23.02.2011
• EAN: 9781934115152
• ISBN: 978-1-934115-15-2
• No. of pages: 278
• Weight: 893 g
• Illustrations: X, 278 p. 56 illus., 7 illus. in color.
• Series: Methods in Molecular Biology; Methods in Molecular Biology
• Subjects: Natural sciences, medicine, IT, technology > Medicine > Clinical medicine; Diabetes, B, Medical research, INTERNAL MEDICINE, Endocrinology, molecular biology, Biomedical and Life Sciences, Biomedical Research, Molecular Medicine