Essential Zebrafish Methods - Cell and Developmental Biology

Informationen zum Autor Professor, Department of Biology, Institute of Neuroscience, University of Oregon, Eugene, OR, USA Professor of Biochemistry and Marine Biology at Northeastern University, promoted 1996. Joined Northeastern faculty in 1987. Previously a faculty member in Dept. of Biochemistry at the University of Mississippi Medical Center, 1983-1987.Principal Investigator in the U.S. Antarctic Program since 1984. Twelve field seasons "on the ice" since 1981. Research conducted at Palmer Station, Antarctica, and McMurdo Station, Antarctica.Research areas: Biochemical, cellular, and physiological adaptation to low and high temperatures. Structure and function of cytoplasmic microtubules and microtubule-dependent motors from cold-adapted Antarctic fishes. Regulation of tubulin and globin gene expression in zebrafish and Antarctic fishes. Role of microtubules in morphogenesis of the zebrafish embryo. Developmental hemapoiesis in zebrafish and Antarctic fishes. UV-induced DNA damage and repair in Antarctic marine organisms. Klappentext Due to its prolific reproduction and the external development of the transparent embryo, the zebrafish is the prime model for genetic and developmental studies, as well as research in genomics. While genetically distant from humans, nonetheless the vertebrate zebrafish has comparable organs and tissues which make it the model organism for study of vertebrate development. This book will provide overview of commonly used methods and a comprehensive collection of protocols describing the most powerful techniques. The methods and techniques in this volume were chosen by the editors of Methods in Cell Biology, whose goal was to provide fail-safe methods, tips, and "tricks of the trade" to experienced researchers and more junior members in the lab. Inhaltsverzeichnis Overview of the Zebrafish System H. William Detrich III Cell Cycles and Development in the Embryonic Zebrafish Donald A. Kane Primary Fibroblast Cell Culture Barry H. Paw Production of Haploid and Diploid Androgenetic Zebrafish (Including Methodology for Delayed in Vitro Fertilization) John H. Postlethwait Analysis of Protein and Gene Expression Trevor Jowett Analysis of Zebrafish Development Using Explant Culture Assays Hazel Sive Confocal Microscopic Analysis of Morphogenetic Movements Mark S. Cooper Cytoskeletal Dynamics of the Zebrafish Embryo Lilianna Solinica-Krezel Haploid Screens and Gamma-Ray Mutagenesis Charline Walker Positional Cloning of Mutated Zebrafish Genes Will Talbot The Zebrafish Genome John Postlethwait Analyzing Axon Guidance in the Zebrafish Retinotectal System Chi-Bin Chien Analysis of the Cell Cycle in Zebrafish Embryos James F. Amatruda Cellular Dissection of Zebrafish Hematopoiesis David Traver Culture of Embryonic Stem Cell Lines from Zebrafish Paul Collodi Neurogenesis Laure Bally-Cuif Time-Lapse Microscopy of Brain Development Scott E. Fraser Development of the Peripheral Sympathetic Nervous System in Zebrafish Paul D. Henion Approaches to Study Neurogenesis in the Zebrafish Retina Jarema Malicki Instrumentation for Measuring Oculomotor Performance and Plasticity in Larval Organisms David W. Tank Development of Cartilage and Bone Thomas F. Schilling Morphogenesis of the Jaw: Development Beyond the Embryo Pamela C. Yelick Cardiac Development Didier Y. R. Stainier Zebrafish Kidney Development Iain A. Drummond Retroviral-Mediated Insertional Mutogenesis in Zebrafish Adam Amsterdam Genetic Screens for Maternal-Effect Mutations Francisco Pelegri Behavior Screening Assays in Zebrafish Herwig Baier A High-Throughput Method for Identifying N-Ethyl-N-Nitrosourea (ENU)-Induced Point Mutations in Zebrafish Cecelia B. Moens Transgenesis and Gene Trap Methods in Zebrafish by Using the Tol2 Transposable Element Koi...

List of contents

Overview of the Zebrafish System
H. William Detrich III
Cell Cycles and Development in the Embryonic Zebrafish
Donald A. Kane
Primary Fibroblast Cell Culture
Barry H. Paw
Production of Haploid and Diploid Androgenetic Zebrafish (Including Methodology for Delayed in Vitro Fertilization)
John H. Postlethwait
Analysis of Protein and Gene Expression
Trevor Jowett
Analysis of Zebrafish Development Using Explant Culture Assays
Hazel Sive
Confocal Microscopic Analysis of Morphogenetic Movements
Mark S. Cooper
Cytoskeletal Dynamics of the Zebrafish Embryo
Lilianna Solinica-Krezel
Haploid Screens and Gamma-Ray Mutagenesis
Charline Walker
Positional Cloning of Mutated Zebrafish Genes
Will Talbot
The Zebrafish Genome
John Postlethwait
Analyzing Axon Guidance in the Zebrafish Retinotectal System
Chi-Bin Chien
Analysis of the Cell Cycle in Zebrafish Embryos
James F. Amatruda
Cellular Dissection of Zebrafish Hematopoiesis
David Traver
Culture of Embryonic Stem Cell Lines from Zebrafish
Paul Collodi
Neurogenesis
Laure Bally-Cuif
Time-Lapse Microscopy of Brain Development
Scott E. Fraser
Development of the Peripheral Sympathetic Nervous System in Zebrafish
Paul D. Henion
Approaches to Study Neurogenesis in the Zebrafish Retina
Jarema Malicki
Instrumentation for Measuring Oculomotor Performance and Plasticity in Larval Organisms
David W. Tank
Development of Cartilage and Bone
Thomas F. Schilling
Morphogenesis of the Jaw: Development Beyond the Embryo
Pamela C. Yelick
Cardiac Development
Didier Y. R. Stainier
Zebrafish Kidney Development
Iain A. Drummond
Retroviral-Mediated Insertional Mutogenesis in Zebrafish
Adam Amsterdam
Genetic Screens for Maternal-Effect Mutations
Francisco Pelegri
Behavior Screening Assays in Zebrafish
Herwig Baier
A High-Throughput Method for Identifying N-Ethyl-N-Nitrosourea (ENU)-Induced Point Mutations in Zebrafish
Cecelia B. Moens
Transgenesis and Gene Trap Methods in Zebrafish by Using the Tol2 Transposable Element
Koichi Kawakami
Molecular Cytogenetic Methodologies and a Bacterial Artificial Chromosome (BAC)Probe Panel Resource for Genomic Analysis in Zebrafish
Charles Lee
The Zon Laboratory Guide to Positional Cloning in Zebrafish
Leonard I. Zon
Sleeping Beauty Transposon for Efficient Gene Delivery
Stephen C. Ecker
Transgene Manipulation in Zebrafish by Using Recombinases
Gary W. Stuart
Highly Efficient Zebrafish Transgenesis Mediated by the Meganuclease I-SceI
Joachim Wittbrodt
Cloning Zebrafish by Nuclear Transfer
Shuo Lin
Spatial and Temporal Expression of the Zebrafish Genome by Large-Scale In Situ Hybridization Screening
Christine Thisse
Genetic Backgrounds, Standard Lines, and Husbandry of Zebrafish
Bill Trevarrow
Common Diseases of Laboratory Zebrafish
Jennifer L. Matthews
Zebrafish Sperm Cryopreservation
Thomas Look

Product details

• Authors: H. William Detrich, Westerfield, Monte Westerfield, Leonard I. Zon
• Assisted by: H. William Detrich (Editor), III Detrich (Editor), H William Detrich III (Editor), H. Detrich III (Editor), H. William Detrich III (Editor), H. William Detrich III (Editor), Monte Westerfield (Editor), Westerfield Monte (Editor), Leonard I Zon (Editor), Leonard I. Zon (Editor), Zon Leonard I. (Editor)
• Publisher: ELSEVIER SCIENCE BV
• Languages: English
• Product format: Paperback / Softback
• Released: 09.10.2009
• EAN: 9780123745996
• ISBN: 978-0-12-374599-6
• No. of pages: 584
• Series: Reliable Lab Solutions; Reliable Lab Solutions
• Subjects: Natural sciences, medicine, IT, technology > Biology > General, dictionaries; SCIENCE / Life Sciences / Cell Biology, Cellular biology (cytology)