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In recent years Chlamydomonas reinhardtii (C. reinhardtii) has proved to be an outstanding model for investigation of signal transduction, rhodopsin-based vision, and the evolution of sexual processes. The Chlamydomonas Sourcebook, first published in 1989, summarized the development of this alga as a laboratory system beginning in the 1940s. Since the first edition published, Chlamydomonas research has grown significantly. This revised three-volume set, which includes the Sourcebook, Chlamydomonas Metabolic Processes, and Chlamydomonas Motility and Taxis Responses stands as the most comprehensive reference for this important research organism. This set retains historical material, and culture methods and illustrations from the original book, while adding molecular techniques, analysis of the recently sequenced genome, and reviews of the current status of the diverse fields in which Chlamydomonas is used as a model organism. * Edited by the leading researcher in chlamydomonas science * Includes best practices for applications in research, including methods for culture, preservation of cultures, preparation of media, lists of inhibitors, and more * Aids researchers with common laboratory problems such as contamination and details on properties of particular strains and mutants * The latest advances in research including completion of the genome * A broad perspective for studies in cell and molecular biology, genetics, plant physiology and related fields * Companion Website with videos that illustrate the marvels of the Chlamydomonas flagella in action
List of contents
VOLUME 1 - Introduction to Chlamydomonas and Its Laboratory Use
By Elizabeth Harris (Duke University)
. Taxonomy, diversity and distribution of Chlamydomonas species
. History of Chlamydomonas as a laboratory organism
. Cell architecture
. The vegetative cell cycle, including culture conditions and methods
. The sexual cycle
. Overview of the nuclear genome
. Genetic analysis
. The molecular toolkit, including transformation, gene expression, etc.
. Resources for the investigator and for use of Chlamydomonas in teaching
VOLUME 2 - Organellar and Metabolic Processes
Edited By David Stern (Boyce Thompson Institute for Plant Research)
. Biogenesis of chloroplasts and mitochondria
. Photosynthesis
. Respiration
. Nitrogen assimilation
. Starch biosynthesis
. Sulfur metabolism
. Rsponse to heavy metals
. Hydrogen production
VOLUME 3 - Cell Motility and Behavior
Edited by George Witman (University of Massachusetts Medical School)
. Basal bodies
. Basal body-associated structures
. Flagellar assembly and length control
. Intraflagellar transport
. Flagellar axoneme
. Flagellar membrane
. Tactic responses (phototaxis, chemotaxis) and mechanoreception
