Macromolecular Drug Delivery - Methods and Protocols

Macromolecular drugs hold the promise of becoming new therapeutics for several major disorders, including cancer and cardiovascular disease. This incredible potential is explored in Macromolecular Drug Delivery, a volume which gives a wide-ranging overview of contemporary methods used in the field, and which addresses the limitations presented by a lack of safe and efficient drug delivery strategies. Chapters offer information on both in vitro and in vivo methods of macromolecular delivery, thus appealing to a broad scientific audience. Composed in the highly successful Methods in Molecular Biology(TM) series format, each chapter contains a brief introduction, step-by-step methods, a list of necessary materials, and a Notes section which shares tips on troubleshooting and avoiding known pitfalls.
Comprehensive and cutting-edge, Macromolecular Drug Delivery offers a platform for interdisciplinary collaboration, which should provide opportunities for new discoveries at the interface between disciplines. Ultimately, this cooperation will lead to the use of macromolecular drugs as novel diagnostic tools and, even more importantly, as a means to revolutionize the way we view and treat diseases.

List of contents

Developments in Macromolecular Drug Delivery.- Synthetic vs. Natural/Biodegradable Polymers for Delivery of shRNA-Based Cancer Therapies.- siRNA and DNA Transfer to Cultured Cells.- Poly(?-amino esters): Procedures for Synthesis and Gene Delivery.- Systemic Delivery and Pre-clinical Evaluation of Nanoparticles Containing Antisense Oligonucleotides and siRNAs.- Peptide-Based Delivery of Steric-Block PNA Oligonucleotides.- Characterizing Peptide-Mediated DNA Internalization in Human Cancer Cells.- Selection and Characterization of Antibodies from Phage Display Libraries Against Internalizing Membrane Antigens.- Artificial Membrane Models for the Study of Macromolecular Delivery.- Enhanced Delivery of Macromolecules into Cells by Electroendocytosis.- In Vitro Systems for Studying Epithelial Transport of Macromolecules.- Preparation of Macromolecule-Containing Dry Powders for Pulmonary Delivery.- Macromolecular Delivery Across the Blood-Brain Barrier.- Positron Emission Tomography (PET) and Macromolecular Delivery In Vivo.

Summary

Macromolecular drugs hold the promise of becoming new therapeutics for several major disorders, including cancer and cardiovascular disease. This incredible potential is explored in Macromolecular Drug Delivery, a volume which gives a wide-ranging overview of contemporary methods used in the field, and which addresses the limitations presented by a lack of safe and efficient drug delivery strategies. Chapters offer information on both in vitro and in vivo methods of macromolecular delivery, thus appealing to a broad scientific audience. Composed in the highly successful Methods in Molecular Biology™ series format, each chapter contains a brief introduction, step-by-step methods, a list of necessary materials, and a Notes section which shares tips on troubleshooting and avoiding known pitfalls.
Comprehensive and cutting-edge, Macromolecular Drug Delivery offers a platform for interdisciplinary collaboration, which should provide opportunities for new discoveries at the interface between disciplines. Ultimately, this cooperation will lead to the use of macromolecular drugs as novel diagnostic tools and, even more importantly, as a means to revolutionize the way we view and treat diseases.

Additional text

From the reviews:

"This volume … provides details and discussions on contemporary methods available to address the limitations in the delivery of these agents. … This comprehensive review will be of interest to scientists investigating either the in vitro or in vivo actions of macromolecule agents. Experts in biophysics, chemistry, cell biology, pharmaceutics, and/or preclinical and clinical dug discovery studies will find this book of value. Researchers involved in target therapy strategies for treatment of diseases will want a copy of this book." (Thomas L. Pazdernik, Doody’s Review Service, 2009)

“The title of the book will definitely attract readers of various backgrounds. It seems to indicate that the book covers the delivery of various kinds of macromolecular drugs. … This will come as a surprise for experts working with other types of macromolecular therapeutics … . the in vitro and in vivo models described in the book would be of interest to a broad scientific audience. … the book definitely contains a lot of valuable information for experimental researchers.” (Michal Pechar, ChemMedChem, Issue 4, 2009)

Report

From the reviews:

"This volume ... provides details and discussions on contemporary methods available to address the limitations in the delivery of these agents. ... This comprehensive review will be of interest to scientists investigating either the in vitro or in vivo actions of macromolecule agents. Experts in biophysics, chemistry, cell biology, pharmaceutics, and/or preclinical and clinical dug discovery studies will find this book of value. Researchers involved in target therapy strategies for treatment of diseases will want a copy of this book." (Thomas L. Pazdernik, Doody's Review Service, 2009)
"The title of the book will definitely attract readers of various backgrounds. It seems to indicate that the book covers the delivery of various kinds of macromolecular drugs. ... This will come as a surprise for experts working with other types of macromolecular therapeutics ... . the in vitro and in vivo models described in the book would be of interest to a broad scientific audience. ... the book definitely contains a lot of valuable information for experimental researchers." (Michal Pechar, ChemMedChem, Issue 4, 2009)

Product details

• Assisted by: Mattia Belting (Editor), Mattias Belting (Editor)
• Publisher: Springer, Berlin
• Languages: English
• Product format: Hardback
• Released: 23.02.2011
• EAN: 9781588299994
• ISBN: 978-1-58829-999-4
• No. of pages: 205
• Weight: 444 g
• Illustrations: XIV, 205 p.
• Series: Methods in Molecular Biology; Methods in Molecular Biology
• Subjects: Natural sciences, medicine, IT, technology > Medicine > Pharmacy; Biotechnologie, Transport, Pharmazie, Apotheke, B, Pharmacology, Pharmacy, Biomedical and Life Sciences, Pharmacology/Toxicology, Pharmacy / dispensing, Bioprocess Engineering, Biochemical Engineering, Chemical Bioengineering