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Klappentext This volume demonstrates how cellular and associated electron microscopy contributes to knowledge about biological structural information, primarily at the nanometer level. It presents how EM approaches complement both conventional structural biology (at the high end, angstrom level of resolution) and digital light microscopy (at the low end, 100-200 nanometers). Zusammenfassung Suitable for those who may be considering electron microscopy as a tool to extend molecular! biochemical! or light microscope observations! this book shows the advantages of electron microscopy and the protocols required for fine structural visualization of individual molecules upwards! ranging from mammalian tissues to experimental organisms. Inhaltsverzeichnis Scanning Electron Microscopy at the sub-cellular level; Electron Probe X-ray microanalysis for the study of cell physiology; Preparation of cells and tissues for Immuno EM; Fixation, Freezing and Embedding for Genetically Altered Tissue; Combined video fluorescence and 3-D electron microscopy; From Live-cell Imaging to Scanning Electron Microscopy (SEM); Immunolabelling for High Resolution; Immunogoldlabeling of Thawed Cryosections; Close-to-Native Ultrastructural Preservation by High Pressure Freezing; High-Pressure Freezing and Low-Temperature Fixation of Cell Monolayers Grown on Sapphire Coverslips; Freeze-fracture Cytochemistry in Cell Biology; The Plasma membrane cytoskeleton; Correlated Light and Electron Microscopy of the Cytoskeleton; Electron microscopy of intermediate filaments; Studying Microtubules by Electron Microscopy; Endoplasmic reticulum and Golgi Complex; Visualization of Dynamins; Electron Microscopy of Collagen Fibril Structure In Vitro And In Vivo including Three-Dimensional Reconstruction; Visualisation of Desmosomes in the Electron Microscope; A Protocol for Isolation and Visualization of Yeast Nuclei by Scanning Electron Microscopy (SEM); High Pressure Freezing and Freeze Substitution of S.pombe and S.cerevisiae for TEM; Electron Microscopy of Lamin and the Nuclear Lamina in Caenorhabditis elegans ; Visualization of Nuclear Organisation by Ultrastructural Cytochemistry; Scanning Electron Microscopy of Chromosomes; Infection at the Cellular Level; Electron Microscopy of Viruses and Virus-Cell Interactions....
