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In this first comprehensive resource to cover the application of single molecule techniques to biological measurements, the pioneers in the field show how to both set up and interpret a single molecule experiment.Following an introduction to single molecule measurements and enzymology, the expert authors consider molecular motors and mechanical properties before moving on to the applications themselves. Detailed discussions of studies on protein enzymes, ribozymes and nucleic acids are also included.
List of contents
A Road Map to Single Molecule DynamicsSingle Molecule Study for Elucidating the Mechanism used by Biosystems to Utilize Thermal FluctuationsImaging and Molecular MotorsIon ChannelSignal Transduction across the Plasma MembraneDynamics of Membrane Receptors: Single Molecule Tracking of Quantum Dot Liganded Epidermal Growth FactorStudying Dynamics of Ligand-Receptor Complexes by Single-Molecule TechniquesRNA in CellsProtein Dynamics and InteractionsTwo Rotary Motors of ATP SynthaseSingle Molecule FRET Studies of Helicases and Holliday JunctionsHigh-speed Atomic Force Microscopy for Nano-visualization of Biomolecular ProcessesForce-clamp Spectroscopy of Single Proteins
About the author
Toshio Yanagida did his undergraduate and graduate studies in semi-conductor physics at Osaka University (Osaka, Japan). He then switched to biophysics in 1970 to pursue a career in basic science. He is now professor and Dean of the Osaka University Graduate School of Frontier Biosciences, and project leader at both the Single Molecule Processes Project and the Kansai Advanced Research Center Communications in Japan. His teams are leading the development of single-molecule detection techniques to investigate the molecular motor of contracting muscle.Professor Yanagida is a founding editor of the Journals "Single Molecules" and "Small". For his scientific achievements, he has received numerous awards, among them the Japan Academy Award and the Japanese Imperial Award.Yoshiharu Ishii studied physics and biophysicsat the Universities of Tokyo and Nagoya (Japan). From 1983 onwards he worked at the Boston Biomedical Research Institute (USA), returning to Japan in 1992 to pursue his research career in applied biophysics. He is currently a group leader in the soft nanomachine project at Osaka University.
Summary
In this first comprehensive resource to cover the application of single molecule techniques to biological measurements, the pioneers in the field show how to both set up and interpret a single molecule experiment.
Following an introduction to single molecule measurements and enzymology, the expert authors consider molecular motors and mechanical properties before moving on to the applications themselves. Detailed discussions of studies on protein enzymes, ribozymes and nucleic acids are also included.
Report
"An invaluable resource to rapidly familiarize oneself with the diverse approaches to single molecule experimentation." ( The Quarterly Review of Biology , December 2009)
