Methods in Enzymology - Nitric Oxide: Oxidative Nitrosative Stress in Redox Regulation of

Informationen zum Autor Lester Packer received a PhD in Microbiology and Biochemistry in 1956 from Yale University. In 1961, he joined the University of California at Berkeley serving as Professor of Cell and Molecular Biology until 2000, and then was appointed Adjunct Professor, Pharmacology and Pharmaceutical Sciences, School of Pharmacy at the University of Southern California. Dr Packer received numerous distinctions including three honorary doctoral degrees, several distinguished Professor appointments. He was awarded Chevalier de l’Ordre National du Merite (Knight of the French National Order of Merit) and later promoted to the rank of Officier. He served as President of the Society for Free Radical Research International (SFRRI), founder and Honorary President of the Oxygen Club of California. He has edited numerous books and published research; some of the most cited articles have become classics in the field of free radical biology: Dr Packer is a member of many professional societies and editorial boards. His research elucidated - the Antioxidant Network concept. Exogenous lipoic acid was discovered to be one of the most potent natural antioxidants and placed as the ultimate reductant or in the pecking order of the “Antioxidant Network? regenerating vitamins C and E and stimulating glutathione synthesis, thereby improving the overall cellular antioxidant defense. The Antioxidant Network is a concept addressing the cell’s redox status. He established a world-wide network of research programs by supporting and co-organizing conferences on free radical research and redox biology in Asia, Europe, and America. Klappentext The discovery that nitrogen monoxide or nitric oxide (NO)is a biologically produced free radical has revolutionized our thinking about physiological and pathological processes. This discovery has ignited enormous interest in the scientific community. When generated at low levels, NO is a signaling molecule, but at high concentration, NO is a cytotoxic molecule. The physiological and pathological processes of NO production and metabolism and its targets, currently areas of intensive research, have important pharmacologic implications for health and disease. Zusammenfassung Nitric Oxide (NO) is one of the few gaseous signaling molecules and is a key biological messenger that plays a role in many biological processes. This book includes robust methods. It is suitable for researchers interested in the detailed biochemistry of nitric oxide and its synthesis. Inhaltsverzeichnis Chapter One: Protein 3-Nitrotyrosine in Complex Biological Samples: Quantification by High-Pressure Liquid Chromatography/Electrochemical Detection and Emergence of Proteomic Approaches for Unbiased Identification of Modification Sites Chapter Two: Selective Fluorogenic Derivatization of 3-Nitrotyrosine and 3,4-Dihydroxyphenylalanine in Peptides: A Method Designed for Quantitative Proteomic Analysis Chapter Three: Nitroalkenes: Synthesis, Characterization, and Effects on Macrophage Activation Chapter Four: In-Gel Detection of S-Nitrosated Proteins Using Fluorescence Methods Chapter Five: The Arachidonate-Dependent Survival Signaling Preventing Toxicity in Monocytes/Macrophages Exposed to Peroxynitrite Chapter Six: Practical Approaches to Investigate Redox Regulation of Heat Shock Protein Expression and Intracellular Glutathione Redox State Chapter Seven: Monitoring Oxidative Stress in Vascular Endothelial Cells in Response to Fluid Shear Stress: From Biochemical Analyses to Micro- and Nanotechnologies Chapter Eight: Determination of S-Nitrosothiols in Biological and Clinical Samples Using Electron Paramagnetic Resonance Spectrometry with Spin Trapping Chapter Nine: Novel Method for Measuring S-Nitrosothiols Using Hydrogen Sulfide Chapter Ten: Kinetic Studies on Peroxynitrite Reduction by Peroxiredoxins ...

List of contents

Chapter One: Protein 3-Nitrotyrosine in Complex Biological Samples: Quantification by High-Pressure Liquid Chromatography/Electrochemical Detection and Emergence of Proteomic Approaches for Unbiased Identification of Modification Sites
Chapter Two: Selective Fluorogenic Derivatization of 3-Nitrotyrosine and 3,4-Dihydroxyphenylalanine in Peptides: A Method Designed for Quantitative Proteomic Analysis
Chapter Three: Nitroalkenes: Synthesis, Characterization, and Effects on Macrophage Activation
Chapter Four: In-Gel Detection of S-Nitrosated Proteins Using Fluorescence Methods
Chapter Five: The Arachidonate-Dependent Survival Signaling Preventing Toxicity in Monocytes/Macrophages Exposed to Peroxynitrite
Chapter Six: Practical Approaches to Investigate Redox Regulation of Heat Shock Protein Expression and Intracellular Glutathione Redox State
Chapter Seven: Monitoring Oxidative Stress in Vascular Endothelial Cells in Response to Fluid Shear Stress: From Biochemical Analyses to Micro- and Nanotechnologies
Chapter Eight: Determination of S-Nitrosothiols in Biological and Clinical Samples Using Electron Paramagnetic Resonance Spectrometry with Spin Trapping
Chapter Nine: Novel Method for Measuring S-Nitrosothiols Using Hydrogen Sulfide
Chapter Ten: Kinetic Studies on Peroxynitrite Reduction by Peroxiredoxins
Chapter Eleven: Nitrocytochrome c: Synthesis, Purification, and Functional Studies
Chapter Twelve: Tyrosine Nitration, Dimerization, and Hydroxylation by Peroxynitrite in Membranes as Studied by the Hydrophobic Probe N-T-BOC-L-tyrosine tert-Butyl Ester
Chapter Thirteen: Assessment of Superoxide Production and NADPH Oxidase Activity by HPLC Analysis of Dihydroethidium Oxidation Products
Chapter Fourteen: Methods to Measure the Reactivity of Peroxynitrite-Derived Oxidants Toward Reduced Fluoresceins and Rhodamines
Chapter Fifteen: Detection and Characterization of Peroxynitrite-Induced Modifications of Tyrosine, Tryptophan, and Methionine Residues by Tandem Mass Spectrometry
Chapter Sixteen: Reductive Gas-Phase Chemiluminescence and Flow Injection Analysis for Measurement of the Nitric Oxide Pool in Biological Matrices
Chapter Seventeen: Detection and Measurement for the Modification and Inactivation of Caspase by Nitrosative Stress In Vitro and In Vivo
Chapter Eighteen: Interactive Relations between Nitric Oxide (NO) and Carbon Monoxide (CO): Heme Oxygenase-1/CO Pathway Is a Key Modulator in NO-Mediated Antiapoptosis and Anti-inflammation
Chapter Nineteen: Detection and Characterization of In Vivo Nitration and Oxidation of Tryptophan Residues in Proteins
Chapter Twenty: In Vivo Real-Time Measurement of Nitric Oxide in Anesthetized Rat Brain
Chapter Twenty-One: Nitric Oxide and Cardiobiology-Methods for Intact Hearts and Isolated Myocytes
Chapter Twenty-Two: Microscopic Technique for the Detection of Nitric Oxide-Dependent Angiogenesis in an Animal Model
Author Index
Subject Index

Product details

• Authors: Enrique Cadenas, Enrique (EDT)/ Packer Cadenas, Lester Packer
• Assisted by: Enrique Cadenas (Editor), Lester Packer (Editor)
• Publisher: Academic Press London
• Languages: English
• Product format: Hardback
• Released: 26.06.2008
• EAN: 9780123743091
• ISBN: 978-0-12-374309-1
• No. of pages: 436
• Dimensions: 159 mm x 235 mm x 25 mm
• Series: Methods in Enzymology; Methods in Enzymology
• Subjects: Natural sciences, medicine, IT, technology > Biology > Biochemistry, biophysics; SCIENCE / Life Sciences / Biochemistry, biochemistry