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Plant protoplasts have proved to be an excellent tool for in vitro manipulation, somatic hybridization, DNA uptake and genetic trans formation, and for the induction of somac1onal variation. These studies reflect the far-reaching impact of protoplast research in agriculture and forest biotechnology. Taking these aspects into consideration, the series of books on Plant Protoplasts and Genetic Engineering provides a survey of the literature, focusing on recent information and the state of the art in protoplast manipulation and genetic transformation. This book, Plant Protoplasts and Genetic Engineering VII, like the previous six volumes published in 1989, 1993, 1994, and 1995, is unique in its approach. It comprises 27 chapters dealing with the regeneration of plants from protoplasts, and genetic transformation in various species of Agrostis, Allium, Anthriscus, Asparagus, Avena, Boehmeria, Carthamus, Coffea, Funaria, Geranium, Ginkgo, Gladiolus, Helianthus, Hordeum, Lilium, Lithospermum, Mentha, Panax, Papaver, Passiflora, Petunia, Physcomi trella, Pinus, Poa, Populus, Rubus, Saintpaulia, and Swertia. This book may be of special interest to advanced students, teachers, and research scientists in the field of plant tissue culture, molecular biology, genetic engineering, plant breeding, and general biotechnology. New Delhi, June 1996 Professor y. P. S. BAJAJ Series Editor Contents Section I Regeneration of Plants from Protoplasts 1. 1 Regeneration of Plantlets from Protoplasts of Allium cepa (Onion) E. E. HANSEN, J. F. HUBSTENBERGER, and G. C. PHILLIPS (With 3 Figures) 1 Introduction . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3 2 Protoplast Isolation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4 3 Protoplast Culture . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 8 4 Regeneration of Plantlets . . . . . . . . . . . . . . . . . . . . . . . . . . . 9 5 Summary. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
List of contents
Section I Regeneration of Plants from Protoplasts.- I. 1 Regeneration of Plantlets from Protoplasts of Allium cepa (Onion).- I. 2 Regeneration of Plants from Protoplasts of Anthriscus sylvestris (L.) Hoffm. (Woodland Beakchervil).- I. 3 Regeneration of Plants from Protoplasts of Coffea spp. (Coffee).- I. 4 Direct Embryogenesis in Protoplasts of Ginkgo biloba (Maidenhair Tree).- I. 5 Regeneration of Plants from Protoplasts of Helianthus annuus L. (Sunflower).- I. 6 Regeneration of Plants from Protoplasts of Hordeum vulgare (Barley).- I. 7 Regeneration of Plants from Protoplasts of Lilium x formolongi.- I. 8 Regeneration of Plants from Protoplasts of Mentha piperita L. (Peppermint).- I. 9 Regeneration of Plants from Protoplasts of Mosses (Funaria hygrometrica and Physcomitrella patens).- I. 10 Regeneration of Plants from Protoplasts of Passiflora Species (Passion Fruit).- I. 11 Regeneration of Plants from Protoplasts of Poa pratensis L. (Kentucky Blue Grass).- I. 12 Regeneration of Plants from Protoplasts of Populus Species (Poplars).- I. 13 Regeneration of Plants from Protoplasts of Saintpaulia ionantha H. Wendl. (African Violet).- Section II Genetic Transformation and Transgenic Plants.- II. 1 Genetic Transformation in Agrostis palustris Huds. (Creeping Bentgrass).- II. 2 Genetic Transformation in Asparagus officinalis L..- II. 3 Genetic Transformation in Avena sativa L. (Oat).- II. 4 Genetic Transformation in Boehmeria nivea Gaud. (Ramie Fiber).- II. 5 Genetic Transformation of Carthamus tinctorius L. (Safflower).- II. 6 Genetic Transformation of Geraniums.- II. 7 Genetic Transformation of Gladiolus.- II. 8 Genetic Transformation of Lithospermum erythrorhizon for Increased Production of Shikonin.- II. 9 Genetic Transformation in Papaver somniferum L. (Opium Poppy) forEnhanced Production of Morphinan.- II. 10 Genetic Transformation of Panax ginseng (C. A. MEYER) for Increased Production of Ginsenosides.- II. 11 Genetic Transformation in Petunia.- II. 12 Genetic Transformation in Pinus elliottii Engelm. (Slash Pine).- II. 13 Genetic Transformation in Raspberries and Blackberries (Rubus Species).- II. 14 Genetic Transformation in Swertia japonica.
