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Informationen zum Autor William S. Kisaalita, PhD is professor and former coordinator of graduate engineering programs at the University of Georgia, where he also directs the Cellular Bioengineering Laboratory. The main research focus of his laboratory is cell-surface interactions with applications in cell-based biosensing in drug discovery. He has published more than 80 peer reviewed and trade press papers and made more than 100 poster and podium presentations. He has received numerous instructional awards including membership in the University of Georgia Teaching Academy. He is a member of ACS, AAAS, ASEE, and SBS. Dr. Kisaalita serves on the editorial boards of The Open Biotechnology Journal and The Journal of Community Engagement and Scholarship . Klappentext This book is based upon cutting-edge research conducted in the authora (TM)s lab (Cellular Bioengineering)! which over the past decade has developed a number of sophisticated techniques to facilitate use of 3D cell based assays or biosensors. This book uses data from peer-reviewed publications to conclusively justify use of 3D cell cultures in cell-based biosensors (assays) for (HTS). The majority of assays performed in accelerated drug discovery processes are biochemical in nature! but there is a growing demand for live cell-based assays. Unlike biochemical ones! cellular assays are functional approximations of in vivo biological conditions and can provide more biologically relevant information. Zusammenfassung Advances in genomics and combinatorial chemistry inspired innovative technologies and changes in the discovery and pre-clinical development paradigm. This book provides information - from theory to practice - on challenges and opportunities for incorporating 3D cell-based biosensors or assays in drug discovery programs. Inhaltsverzeichnis Introduction. Biosensors and Bioassays. Target-Driven Drug Discovery. 3D versus 2D Cultures. Comparative Genomics and Proteomics. Comparative Structure and Function. Emerging Design Principles. Chemical Microenvironmental Factors. Spatial and Temporal Microenvironmental Factors. Material Physical Property and Force Microenvironmental Factors. Proteomics as a Promising Tool in the Search for 3D Biomarkers. Readout Present and Near Future. Ready-to-Use Commercial 3D Plates. Technology Deployment Challenges and Opportunities. Challenges to Adopting 3D Cultures in HTS Programs. Cases for 3D Cultures in Drug Discovery. Ideal Case Study Design. Appendices. Index. ...
