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For over fifty years the Methods in Enzymology series has been the critically aclaimed laboratory standard and one of the most respected publications in the field of biochemistry. The highly relevant material makes it an essential publication for researchers in all fields of life and related sciences. This volume, the first of three on the topic of Translation Initiation includes articles written by leaders in the field.
List of contents
Chapter 1
Use of Reticulocyte Lysates for Mechanistic Studies of Eukaryotic Translation Initiation
William C. Merrick and Diane Barth-Baus
Chapter 2
Studying translational control in Drosophila cell-free systems
Fátima Gebauer and Matthias W. Hentze
Chapter 3
Use of In Vitro Translation Extract Depleted in Specific Initiation Factors for the Investigation of Translational Regulation
Daniel R. Gallie
Chapter 4
Viral translation and replication in vitro
Yuri V. Svitkin and Nahum Sonenberg
Chapter 5
A practical approach to isolate 48S complexes: Affinity purification and analyses
Nicolas Locker & Peter J. Lukavsky
Chapter 6
Yeast phenotypic assays on translational control
Bumjun Lee, Tsuyoshi Udagawa, Chingakham Ranjit Singh, and Katsura Asano
Chapter 7
Localization and characterization of protein-protein interaction sites
Chingakham Ranjit Singh and Katsura Asano*
Chapter 8
In vivo stabilization of pre-initiation complexes by formaldehyde cross-linking.
Leos Valásek, Bela Szamecz, Alan G. Hinnebusch and Klaus H. Nielsen
Chapter 9
Molecular Genetic Structure-Function Analysis of Translation Initiation Factor eIF5B
Byung-Sik Shin and Thomas E Dever
Chapter 10
The use of fungal in vitro systems for studying translational regulation
Cheng Wu, Nadia Amrani, Allan Jacobson, and Matthew S. Sachs
Chapter 11
Investigating translation initiation using Drosophila molecular genetics
Gritta Tettweiler and Paul Lasko
Chapter 12
Analysis of RNA:protein interactions in vivo: Identification of RNA binding partners of nuclear factor 90
Andrew M. Parrott, Melissa R. Walsh and Michael B. Mathews
Chapter 13
Approaches for Analyzing the Differential Activities and Functions of eIF4E Family Members
Robert E. Rhoads, Tzvetanka D. Dinkova, and Rosemary Jagus
Chapter 14
Tethered function assays: An adaptable approach to study RNA regulatory proteins
Jeff Coller. Mary Wickens
Chapter 15
Analysis of ribosomal shunting during translation initiation in eukaryotic mRNAs
Vincent P. Mauro, Stephen A. Chappell, and John Dresios
Summary
Part of a series which has been the critically acclaimed laboratory standard and one of the respected publications in the field of biochemistry. This volume, on the topic of Translation Initiation, includes articles written by leaders in the field.
