Fr. 220.00

Two-Component Signaling Systems

English · Hardback

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Klappentext Multicellular organisms must be able to adapt to cellular events to accommodate prevailing conditions. Sensory-response circuits operate by making use of a phosphorylation control mechanism known as the "two-component system." Sections include: Structural ApproachesReconstitution of Heterogeneous SystemsIntracellular Methods and AssaysGenome-Wide Analyses of Two-Component Systems Presents detailed protocols Includes troubleshooting tips Zusammenfassung Sensory-response circuits operate by making use of a phosphorylation control mechanism known as the "two-component system." This title features sections including: Structural Approaches; Reconstitution of Heterogeneous Systems; and! Genome-Wide Analyses of Two-Component Systems. It presents protocols and includes troubleshooting tips. Inhaltsverzeichnis Section I: Structural Approaches [1]: The PICM Chemical Scanning Method for Identifying Domain-Domain and Protein-Protein Interfaces: Applications to the Core Signaling Complex of E. coli Chemotaxis [2]: Use of Site-Directed Cysteine and Disulfide Chemistry to Probe Protein Structure and Dynamics: Applications to Soluble and Transmembrane Receptors of Bacterial Chemotaxis [3]: Measuring Distances by Pulsed Dipolar ESR Spectroscopy: Spin-Labeled Histidine Kinases [4]: Rigid Body Refinement of Protein Complexes with Long-Range Distance Restraints from Pulsed Dipolar ESR [5]: TonB/TolA Amino-Terminal Domain Modeling [6]: Functional Dynamics of Response Regulators Using NMR Relaxation Techniques [7]: The Design and Development of Tar-EnvZ Chimeric Receptors [8]: Functional and Structural Characterization of EnvZ, an Osmosensing Histidine Kinase of E. coli [9]: Light Modulation of Histidine-Kinase Activity in Bacterial Phytochromes Monitored by Size Exclusion Chromatography, Crosslinking, and Limited Proteolysis [10]: A Temperature-Sensing Histidine Kinase-Function, Genetics, and Membrane Topology [11]: The Regulation of Histidine Sensor Kinase Complexes by Quorum Sensing Signal Molecules Section II: Reconstitution of Heterogeneous Systems [12]: Liposome-Mediated Assembly of Receptor Signaling Complexes [13]: Analyzing Transmembrane Chemoreceptors Using In Vivo Disulfide Formation Between Introduced Cysteines [14]: Using Nanodiscs to Create Water-Soluble Transmembrane Chemoreceptors Inserted in Lipid Bilayers [15]: Assays for CheC, FliY, and CheX as Representatives of Response Regulator Phosphatases [16]: Genetic Dissection of Signaling Through the Rcs Phosphorelay Section III: Intracellular Methods and Assays [17]: In Vivo Measurement by FRET of Pathway Activity in Bacterial Chemotaxis [18]: In Vivo and In Vitro Analysis of the Rhodobacter sphaeroides Chemotaxis Signaling Complexes [19]: In Vivo Crosslinking Methods for Analyzing the Assembly and Architecture of Chemoreceptor Arrays [20]: A "Bucket of Light? for Viewing Bacterial Colonies in Soft Agar [21]: Phenotypic Suppression Methods for Analyzing Intra- and Inter-Molecular Signaling Interactions of Chemoreceptors [22]: Single-Cell Analysis of Gene Expression by Fluorescence Microscopy Section IV: Genome-Wide Analyses of Two-Component Systems [23]: Two-Component Systems of Mycobacterium tuberculosis-Structure-Based Approaches [24]: Transcriptomic Analysis of ArlRS Two-Component Signaling Regulon, a Global Regulator, in Staphylococcus aureus [25]: Global Analysis of Two-Component Gene Regulation in H. pylori by Mutation Analysis and Transcriptional Profiling [26]: Phosphotransfer Profiling: Systematic Mapping of Two-Component Signal Transduction Pathways and Phosphorelays [27]: Identification of Histidine Phosphorylations in Proteins Using Mass Spectrometry and Affinity-Based Techniques Subject Index Author ...

List of contents

Section I: Structural Approaches
[1]: The PICM Chemical Scanning Method for Identifying Domain-Domain and Protein-Protein Interfaces: Applications to the Core Signaling Complex of E. coli Chemotaxis
[2]: Use of Site-Directed Cysteine and Disulfide Chemistry to Probe Protein Structure and Dynamics: Applications to Soluble and Transmembrane Receptors of Bacterial Chemotaxis
[3]: Measuring Distances by Pulsed Dipolar ESR Spectroscopy: Spin-Labeled Histidine Kinases
[4]: Rigid Body Refinement of Protein Complexes with Long-Range Distance Restraints from Pulsed Dipolar ESR
[5]: TonB/TolA Amino-Terminal Domain Modeling
[6]: Functional Dynamics of Response Regulators Using NMR Relaxation Techniques
[7]: The Design and Development of Tar-EnvZ Chimeric Receptors
[8]: Functional and Structural Characterization of EnvZ, an Osmosensing Histidine Kinase of E. coli
[9]: Light Modulation of Histidine-Kinase Activity in Bacterial Phytochromes Monitored by Size Exclusion Chromatography, Crosslinking, and Limited Proteolysis
[10]: A Temperature-Sensing Histidine Kinase-Function, Genetics, and Membrane Topology
[11]: The Regulation of Histidine Sensor Kinase Complexes by Quorum Sensing Signal Molecules
Section II: Reconstitution of Heterogeneous Systems
[12]: Liposome-Mediated Assembly of Receptor Signaling Complexes
[13]: Analyzing Transmembrane Chemoreceptors Using In Vivo Disulfide Formation Between Introduced Cysteines
[14]: Using Nanodiscs to Create Water-Soluble Transmembrane Chemoreceptors Inserted in Lipid Bilayers
[15]: Assays for CheC, FliY, and CheX as Representatives of Response Regulator Phosphatases
[16]: Genetic Dissection of Signaling Through the Rcs Phosphorelay
Section III: Intracellular Methods and Assays
[17]: In Vivo Measurement by FRET of Pathway Activity in Bacterial Chemotaxis
[18]: In Vivo and In Vitro Analysis of the Rhodobacter sphaeroides Chemotaxis Signaling Complexes
[19]: In Vivo Crosslinking Methods for Analyzing the Assembly and Architecture of Chemoreceptor Arrays
[20]: A "Bucket of Light” for Viewing Bacterial Colonies in Soft Agar
[21]: Phenotypic Suppression Methods for Analyzing Intra- and Inter-Molecular Signaling Interactions of Chemoreceptors
[22]: Single-Cell Analysis of Gene Expression by Fluorescence Microscopy
Section IV: Genome-Wide Analyses of Two-Component Systems
[23]: Two-Component Systems of Mycobacterium tuberculosis-Structure-Based Approaches
[24]: Transcriptomic Analysis of ArlRS Two-Component Signaling Regulon, a Global Regulator, in Staphylococcus aureus
[25]: Global Analysis of Two-Component Gene Regulation in H. pylori by Mutation Analysis and Transcriptional Profiling
[26]: Phosphotransfer Profiling: Systematic Mapping of Two-Component Signal Transduction Pathways and Phosphorelays
[27]: Identification of Histidine Phosphorylations in Proteins Using Mass Spectrometry and Affinity-Based Techniques
Subject Index
Author Index

Product details

Authors Melvin I. (EDT)/ Crane Simon
Assisted by Alexandrine Crane (Editor), Brian Crane (Editor), Brian R. Crane (Editor), Melvin I. Simon (Editor)
Publisher Academic Press London
 
Languages English
Product format Hardback
Released 03.07.2007
 
EAN 9780123738523
ISBN 978-0-12-373852-3
No. of pages 609
Dimensions 157 mm x 241 mm x 44 mm
Series Academic Press
Methods in Enzymology
Academic Press
Methods in Enzymology
Subjects Natural sciences, medicine, IT, technology > Biology > Biochemistry, biophysics

SCIENCE / Life Sciences / Biochemistry, biochemistry, Enzymology

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