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Plant diseases can have an enormous impact on our lives. In a world where total crop failure can quickly lead to human misery and starvation, accurate diagnostics play a key role in keeping plants free from pathogens. In Plant Pathology: Techniques and Protocols, expert researchers provide methods which are vital to the diagnosis of plant diseases across the globe, addressing all three categories of plant pathology techniques: traditional, serological, and nucleic acid. Chapters examine recent and developing issues with crop identity and authenticity, allowing workers to genotype samples from two major food groups. Composed in the highly successful Methods in Molecular Biology(TM) series format, each chapter contains a brief introduction, step-by-step methods, a list of necessary materials, and a Notes section which shares tips on troubleshooting and avoiding known pitfalls.
Authoritative and reader-friendly, Plant Pathology: Techniques and Protocols is an incredible guide which will soon prove to be indispensable, both to novices and expert researchers alike.
List of contents
Bioassay for Diagnosis of Plant Viruses.- Development of Enzyme Linked, Tissue Blot and Dot Blot Immunoassays for Plant Virus Detection.- Immunisation strategies for Antibody Production.- Preparation of Immunogens and Production of Antibodies.- Enzyme-Linked Immunosorbent Assay Detection of Ralstonia solanacearum in Potatoes: The South African Experience.- Production of Monoclonal Antibodies to Plant Pathogens.- Enzyme-Linked Immunosorbent Assay for the Detection and Identification of Plant Pathogenic Bacteria (In Particular for Erwinia amylovora and Clavibacter michiganensis subsp. sepedonicus).- Indirect Immunofluorescence Microscopy for the Detection and Identification of Plant Pathogenic Bacteria (In Particular for Ralstonia solanacearum).- Detection of Four Major Bacterial Potato Pathogens.- Erwinia amylovora: Modern Methods for Detection and Differentiation.- The use of Fluorescent in situ Hybridisation in Plant Fungal Identification and Genotyping.- Use of Molecular Methods for the Detection of Fungal Spores.- Identification of Phytophthora fragariae var. rubi by PCR.- Detection of Double-Stranded RNA Elements in the Plant Pathogenic Fungus Rhizoctonia solani.- Immunocapture-PCR for Plant Virus Detection.- Multiplex Polymerase Chain Reaction (PCR) and Real-time Multiplex PCR for the Simultaneous Detection of Plant Viruses.- Fluorescent-based Techniques for Viral Detection, Quantification andCharacterisation.- Analysis of Population Structures of Viral Isolates using Single StrandConformation Polymorphism (SSCP) Method.- Direct Detection of Plant Viruses in Potato Tubers using Real-time PCR.- An Accelerated Soil Bait Assay for the Detection of Potato Mop Top Virus in Agricultural Soil.- Detection of Phytoplasmas of Temperate Fruit Trees.- PCR Detection of Potato Cyst Nematode.- Potato Cultivar Genome Analysis.- Barley Variety Identification Using SSRs.
Summary
Plant diseases can have an enormous impact on our lives. In a world where total crop failure can quickly lead to human misery and starvation, accurate diagnostics play a key role in keeping plants free from pathogens. In Plant Pathology: Techniques and Protocols, expert researchers provide methods which are vital to the diagnosis of plant diseases across the globe, addressing all three categories of plant pathology techniques: traditional, serological, and nucleic acid. Chapters examine recent and developing issues with crop identity and authenticity, allowing workers to genotype samples from two major food groups. Composed in the highly successful Methods in Molecular Biology™ series format, each chapter contains a brief introduction, step-by-step methods, a list of necessary materials, and a Notes section which shares tips on troubleshooting and avoiding known pitfalls.
Authoritative and reader-friendly, Plant Pathology: Techniques and Protocols is an incredible guide which will soon prove to be indispensable, both to novices and expert researchers alike.
Additional text
From the reviews:
"Plant Pathology: Techniques and Protocols provides a collection of methods for the diagnosis and detection of plant pathogens. … the book can also be used to troubleshoot particular techniques and adapt it to any organism of interest. The notes at the end of each chapter are of great utility … . It is a highly practical book and both students and researchers interested or working on detection and diagnosis will find it very useful." (Arantza Rico, Microbiology Today, November, 2009)
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From the reviews:
"Plant Pathology: Techniques and Protocols provides a collection of methods for the diagnosis and detection of plant pathogens. ... the book can also be used to troubleshoot particular techniques and adapt it to any organism of interest. The notes at the end of each chapter are of great utility ... . It is a highly practical book and both students and researchers interested or working on detection and diagnosis will find it very useful." (Arantza Rico, Microbiology Today, November, 2009)
