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Informationen zum Autor Stephen Sullivan: Trinity College, Dublin, Ireland Kevin Eggan: Harvard University, Cambridge, MA, USA Chad A. Cowan: Harvard University, Cambridge, MA, USA Klappentext Since their isolation in 1998, human embryonic stem (ES) cells have been used as a powerful experimental model for studying the mechanisms of stem cell self-renewal, pluripotency, and differentiation in humans. However, the protocols by which these cells are grown still differ considerably from laboratory to laboratory and standardisation is needed in this young area of research.Human Embryonic Stem Cells: The Practical Handbook contains all basic methods for sourcing, deriving, culturing, and manipulating human ES cells. The editors have gathered the leading scientists in the field to describe the protocols that they have carried out and tested in their own labs.The book* Contains practical information for newcomers to the field: from how to source human ES cells from international cell banks to organizing the laboratory to accommodate work with these cells.* Provides protocols for routine culture as well as specialized protocols for directing differentiation into specific cell lineages.* Addresses the different proclivities and behaviours of individual human ES cell lines: authors state which lines they tried their protocols on and, more importantly, which lines worked best.* Includes troubleshooting sections that address common problems and offer advice on how these can be overcome.* Follows a simple, concise, and consistent structure: background material and hypothesizing are kept to a minimum to insure clarity and usability at the bench.Human Embryonic Stem Cells: The Practical Handbook is the essential source of up-to-date, practical information for all researchers and clinicians working with human embryonic stem cells. Zusammenfassung Written by professionals working in the leading research laboratories, Human Embryonic Stem Cells: The Practical Handbook is a valuable, practical guide to deriving and culturing these cells. The book contains the first centralized collection of methods used in human embryonic stem cell biology. Inhaltsverzeichnis Foreword. Davor Solter Preface. Section 1: Obtaining and culturing human embryonic stem cells. 1 Organization and good aseptic technique in the human embryonic stem cell laboratory. Minal J Patel, Emma L Stephenson, and Stephen L Minger 2 Sourcing human embryonic stem cell lines. Glyn Stacey 3 Human embryo culture for human embryonic stem cell derivation. R. Douglas Powers and Jeannine Witmyer 4 Derivation of human embryonic stem cell lines. Stephen Sullivan, Dieter Egli, Hidenori Akutsu, Douglas A. Melton, Kevin Eggan and Chad A. Cowan 5 Standard culture of human embryonic stem cells. Jeremy M. Crook, Rachel Horne, and Alan Colman 6 Culture of human embryonic stem cells in chemically defined conditions. Julie Hsu Clark and Sheng Ding Section 2: Characterization of undifferentiated embryonic stem cells. 7 Phenotypic analysis of human embryonic stem cells. Jonathan S. Draper, Cheryle A. Séguin and Peter W. Andrews 8 Genetic and epigenetic analysis of human embryonic stem cells. Laurie A. Boyer, Rudolf Jaenisch, and Maisam Mitalipova Section 3: Manipulation of human embryonic stem cells. 9 In vivo differentiation of human embryonic stem cells. Scott A. Noggle, Francesca M. Spagnoli, Ali H. Brivanlou 10 In vitro differentiation of human embryonic stem cells. A. Henry Sathananthan and Alan Trounson 11 Differentiation of human embryonic stem cells into extraembryonic cell types. Cheryle A. Séguin and Jonathan S. Draper 12 Directed differentiation of human embryonic stem cells into: (a) Early endoderm cells. Kenji ...
