Read more
Informationen zum Autor MAHMOUD H. HAMDAN , PhD, FInstP, is the head of the Mass Spectrometry and Separation Technologies Laboratory at GlaxoSmithKline in Verona, Italy. He is a coauthor of Proteomics Today: Protein Assessment and Biomarkers Using Mass Spectrometry , 2D Electrophoresis , and Microarray Technology , also from Wiley. Klappentext Tools, techniques, and progress in cancer biomarkers discoveryThe completion of a number of gene sequencing projects, recent advances in genomic and proteomic technologies, and the availability of powerful bioinformatics tools have led to promising new avenues and approaches in the search for cancer biomarkers. This book provides a comprehensive overview of current methodologies and technologies. It discusses biomarker discovery as a whole, rather than focusing on one specific marker or cancer. With information on both existing and potential biomarkers, Cancer Biomarkers: Analytical Techniques for Discovery:* Provides insights into the current technological platforms for biomarker discovery, including mass spectrometry combined with multidimensional chromatography, DIGE, and various chip technologies* Includes a detailed discussion of protein networks and protein phosphorylation in cancer* Details the use of imaging mass spectrometry, laser capture microdissection, serial analysis of gene expression, enzyme-linked immunosorbent assays, protein microarrays, antibody-based microarrays, and bioinformatics* Covers the emerging role of surface-enhanced laser desorption ionization (SELDI) and various tagging and labeling strategies* Discusses related regulatory and ethical issuesWith a wealth of information that can be applied to a broad spectrum of biomarker research projects, this is a core reference for biomarker researchers, scientists working in proteomics and bioinformatics, pharmaceutical scientists, oncologists, biochemists, biologists, and chemists. Zusammenfassung Tools, techniques, and progress in cancer biomarkers discovery The completion of a number of gene sequencing projects, recent advances in genomic and proteomic technologies, and the availability of powerful bioinformatics tools have led to promising new avenues and approaches in the search for cancer biomarkers. Inhaltsverzeichnis Preface. Acknowledgments. Introduction. 1 Overview. 1.1. Introduction. 1.2. Cancer Biomarkers. 1.3. Phases of Biomarkers Development. 1.4. New Approach to Biomarkers Discovery. 1.4.1. New and Powerful Technologies. 1.4.2. Promising Sources for Biomarkers. 1.4.2.1. DNA Methylation. 1.4.2.2. Mitochondrial DNA Mutations. 1.4.2.3. Phosphatidylinositol-3 Kinases (PI3Ks). 1.4.2.4. Profi ling Tyrosine Phosphorylation. 1.4.2.5. Proteins Expression. 1.5. Initiatives Relevant to Biomarkers Discovery. 1.5.1. Initiatives of the Human Proteome Organization (HUPO). 1.5.2. Data Mining in Cancer Research. 1.6. Concluding Remarks. References. 2 Proteomic Platforms for Biomarkers Discovery. 2.1. Surface Enhanced Laser Desorption Ionization. 2.1.1. Some Basic Considerations. 2.1.2. Protein Capture Surfaces. 2.1.3. Enrichment/prefractionation Prior to SELDI Analysis. 2.1.3.1. Combinatorial Affi nity. 2.1.3.2. Magnetic Beads. 2.1.3.3. Stacked Sorbents. 2.1.3.4. Organic Solvent Extraction. 2.2. Bioinformatics in SELDI. 2.3. Some Representative SELDI Applications. 2.3.1. Addressing Reproducibility in SELDI Analysis. 2.3.2. Limitations and Other Open Questions Regarding Current SELDI. 2.3.3. Other Open Questions. 2.3.4. Outlook. 2.4. Two-dimensional Polyacrylamide Gel Electrophoresis. 2.4.1. Sample Preparation. 2.4.2. Reducing Sample Complexity. 2.4.3. Vari...
