Hplc - A Practical User''s Guide

Informationen zum Autor MARVIN C. MCMASTER, PHD, is a consultant as well as an Adjunct Professor of Chemistry at the University of Missouri? St. Louis. Dr. McMaster has served as a researcher and product developer for DuPont, Kraft Foods, and Ciba-Geigy. Among his other publications, Dr. McMaster is author of LC/MS: A Practical User's Guide and coauthor of GC/MS: A Practical User's Guide (both published by Wiley). Klappentext Everything you need to set up an HPLC systemthat takes advantage of the latest innovationsHere is the perfect handbook for both novice and experienced chromatographers. For novices, HPLC, Second Edition cuts through the technical detail, theory, and confusing variety of column types, enabling them to easily set up and maintain an HPLC system. For more experienced practitioners, the book explains how to fully exploit the most recent innovations and improvements in column technology. The latest information and techniques in high-temperature, micro-flow, and ultra-fast chromatography, as well as the linking of an HPLC to a mass spectrometer, represent just some of the new material featured in this Second Edition.Readers benefit from the author's twenty-five years of experience in HPLC, first as a user, then as a field sales and application support representative, and finally as a teacher and consultant. The book's wealth of tips and troubleshooting advice originate from the author's own laboratory and field work.HPLC's structure is carefully designed to give readers a solid foundationin the basics and then methodically build and refine their skills:* Part I, HPLC Primer, presents the essentials needed to get an HPLC system up and running* Part II, HPLC Optimization, explains how to make the best use of the common columns and troubleshoot problems* Part III, HPLC Utilization, focuses on real-world applications, including interfacing HPLC systems with computers and robotic workstationsA CD-ROM with the author's PowerPoint(r) presentation, Practical Course in HPLC, is included with the book. Plus, new appendices offer answers to frequently asked questions, tables of solvents and volatile buffers, an updated glossary of terms, and laboratory experiments. In short, everything that you need to know to confidently separate, identify, purify, and quantify compounds is here. Zusammenfassung This Second Edition of the classic handbook details how to set up an HPLC system that capitalizes on the latest innovations. It covers new techniques in high-temperature, micro-flow, and ultra-fast chromatography, the linking of an HPLC to a mass spectrometer, and more. Inhaltsverzeichnis Preface xi I HPLC Primer 1 1 Advantages and Disadvantages of HPLC 3 1.1 How It Works 4 1.1.1 A Separation Model of the Column 5 1.1.2 Basic Hardware: A Quick, First Look 7 1.1.3 Use of Solvent Gradients 8 1.1.4 Ranges of Compounds 9 1.2 Other Ways to Make My Separation 9 1.2.1 FPLC-Fast Protein Liquid Chromatography 10 1.2.2 LC-Traditional Liquid Chromatography 10 1.2.3 GLC-Gas Liquid Chromatography 11 1.2.4 SFC-Supercritical Fluid Chromatography 11 1.2.5 TLC-Thin Layer Chromatography 12 1.2.6 EP-Electrophoresis 12 1.2.7 CZE-Capillary Zone Electrophoresis 13 2 Selecting an HPLC System 15 2.1 Characteristic Systems 16 2.1.1 Finding a Fit: Detectors and Data Processing 16 2.1.2 System Models: Gradient Versus Isocratic 16 2.1.3 Vendor Selection 17 2.1.4 Brand Names and Clones 17 2.1.5 Hardware-Service-Support 18 2.2 System Cost Estimates 19 2.2.1 Type I System-QC Isocratic (Cost: $10-15,000) 19 2.2.2 Type II System-Research Gradient (Cost: $20-25,000) 19 2.2.3 Type III System-Automated Clinical (Cost: $25-35,000) 20 2.2.4 Type IV System-Automated Methods (Cost: $30-5...

List of contents

PREFACE.
 
I HPLC PRIMER.
 
1 Advantages and Disadvantages of HPLC.
 
1.1 How It Works.
 
1.2 Other Ways to Make My Separation.
 
2 Selecting an HPLC System.
 
2.1 Characteristic Systems.
 
2.2 System Cost Estimates.
 
2.3 Columns.
 
3 Running Your Chromatograph.
 
3.1 Set-up and Start-up.
 
3.2 Sample Preparation and Column Calibration.
 
3.3 Your First Chromatogram.
 
II HPLC OPTIMIZATION.
 
4 Separation Models.
 
4.1 Partition.
 
4.2 Ion Exchange Chromatography.
 
4.3 Size Exclusion Chromatography.
 
4.4 Affinity Chromatography.
 
5 Column Preparation.
 
5.1 Column Variations.
 
5.2 Packing Materials and Hardware.
 
5.3 Column Selection.
 
6 Column Aging, Diagnosis, and Healing.
 
6.1 Packing Degrading--Bonded-Phase Loss.
 
6.2 Dissolved Packing Material--End Voids.
 
6.3 Bound Material.
 
6.4 Pressure Increases.
 
6.5 Column Channeling--Center-Voids.
 
6.6 Normal Phase, Ion Exchange, and Size Columns.
 
6.7 Zirconium and Polymer Columns.
 
7 Partition Chromatography Modifications.
 
7.1 Reverse-Phase and Hybrid Silica.
 
7.2 Acidic Phase Silica.
 
7.3 Reverse-Phase Zirconium.
 
7.4 Partition Mode Selection.
 
8 "Nonpartition" Chromatography.
 
8.1 Ion Exchange.
 
8.2 Size Exclusion.
 
8.3 Affinity Chromatography.
 
9 Hardware Specifics.
 
9.1 System Protection.
 
9.2 Pumping.
 
9.3 Injectors and Autosamplers.
 
9.4 Detectors.
 
9.5 Fraction Collectors.
 
9.6 Data Collection and Processing.
 
10 Troubleshooting and Optimization.
 
10.1 Hardware and Tools--System Pacification.
 
10.2 Reverse Order Diagnosis.
 
10.3 Introduction to Data Acquisition.
 
10.4 Solvent Conservation.
 
III HPLC UTILIZATION.
 
11 Preparative Chromatography.
 
11.1 Analytical Preparative.
 
11.2 Semipreparative.
 
11.3 "True" Preparative.
 
12 Sample Preparation and Methods Development.
 
12.1 Sample Preparation.
 
12.2 Methods Development.
 
12.3 Gradient Development.
 
13 Application Logics: Separations Overview.
 
13.1 Fat-Soluble Vitamins, Steroid, and Lipids.
 
13.2 Water-Soluble Vitamins, Carbohydrates, and Acids.
 
13.3 Nucleomics.
 
13.4 Proteomics.
 
13.5 Clinical and Forensic Drug Monitoring.
 
13.6 Pharmaceutical Drug Development.
 
13.7 Environmental and Reaction Monitoring.
 
13.8 Application Trends.
 
14 Automation.
 
14.1 Analog-to-Digital Interfacing.
 
14.2 Digital Information Exchange.
 
14.3 HPLC System Control and Automation.
 
14.4 Data Collection and Interpretation.
 
14.5 Automated Methods Development.
 
14.6 Data Exportation to the Real World.
 
15 Recent Advances in LC/MS Separations.
 
15.1 A LC/MS Primer.
 
15.2 Microflow Chromatography.
 
15.3 Ultrafast HPLC Systems.
 
15.4 Chip HPLC Systems.
 
15.5 Standardized LC/MS in Drug Design.
 
16 New Directions in HPLC.
 
16.1 Temperature-Controlled Chromatography.
 
16.2 Ultrafast Chromatography.
 
16.3 Monolith Capillary Columns.
 
16.4 Micro-Parallel HPLC Systems.
 
16.5 Two-Dimensional HPLC Systems.
 
16.6 The Portable LC/MS.
 
APPENDICES.
 
APPENDIX A Personal Separations Guide.
 
AP

Product details

• Authors: Mcmaster, Marvin McMaster, Marvin C McMaster, Marvin C. McMaster
• Publisher: Wiley, John and Sons Ltd
• Languages: English
• Product format: Hardback
• Released: 12.01.2007
• EAN: 9780471754015
• ISBN: 978-0-471-75401-5
• No. of pages: 256
• Dimensions: 165 mm x 245 mm x 20 mm
• Subject: Natural sciences, medicine, IT, technology > Medicine > Pharmacy