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Informationen zum Autor Kiichi Fukui, Tatsuo Ushiki Klappentext Reviewing new trends and techniques for future chromosome research! this volume draws upon scientists from many disciplines to introduce various nano devices and applications that have been developed for handling the smallest biological materials! especially chromosomes. It details the visualization of chromosomes at nano and micro levels! including the most advanced applications ever employed for Scanning Probe Microscopy; discusses the development of a sustainable chromosome imaging database; examines isolation! comparative proteome analyses! and structural/functional analyses including a new 3-dimensional model; and describes a new antibody production method for chromosomal proteins. Zusammenfassung Reviewing trends and techniques for chromosome research, this volume draws upon scientists from various disciplines to introduce various nano devices and applications that have been developed for handling the smallest biological materials, especially chromosomes. It details the visualization of chromosomes at nano and micro levels. Inhaltsverzeichnis Preface. Mechanical Approaches to Elucidate Mechanisms of Chromosome Condensation at the Nano- and Micro-Level. Development of Novel AFM Probes for Chromosome Manipulation. Microchamber Array-Based Sequence-Specific DNA Detection from a Single Chromosome via Trace Volume PCR. On-Chip Chromosome Sorter Using Electric and Magnetic Fields. Fluorescence Microscopy for Analysis of Chromosome Dynamics. Fluorescence In Situ Hybridization (FISH) as a Tool for Comparative Genomics. Immunocytochemistry for Analyzing Chromosomes. Transmission and Scanning Electron Microscopy of Mammalian Metaphase Chromosomes. Atomic Force Microscopy of Human Chromosomes in Relation to Their Higher-Order Structure. Mechanism of Higher-Order Chromatin Folding Revealed by AFM Observation of In Vitro Reconstituted Chromatin. Scanning Near-Field Optical/Atomic Force Microscopy as a Tool for Simultaneous Specification of Chromosome Topography and Particular Gene Location on the Nanometer Scale. Isolation of Human and Plant Chromosomes as Nano-Materials. Proteome Analysis of Human Metaphase Chromosomes. Anti-Peptide Antibodies for Examining the Conformation and Molecular Assembly of an Intracellular Protein. Structure and Interactions of the Imitation SWI-Type Chromatin-Remodeling Complex, ATP-Dependent Chromatin-Assembly Factor. Dynamic and Functional Analysis of Chromosomal Proteins. Development of a Sustainable Chromosome Imaging Database. Image Database and Image Analysis of Chromosome Information. ...
List of contents
Preface. Mechanical Approaches to Elucidate Mechanisms of Chromosome Condensation at the Nano- and Micro-Level. Development of Novel AFM Probes for Chromosome Manipulation. Microchamber Array-Based Sequence-Specific DNA Detection from a Single Chromosome via Trace Volume PCR. On-Chip Chromosome Sorter Using Electric and Magnetic Fields. Fluorescence Microscopy for Analysis of Chromosome Dynamics. Fluorescence In Situ Hybridization (FISH) as a Tool for Comparative Genomics. Immunocytochemistry for Analyzing Chromosomes. Transmission and Scanning Electron Microscopy of Mammalian Metaphase Chromosomes. Atomic Force Microscopy of Human Chromosomes in Relation to Their Higher-Order Structure. Mechanism of Higher-Order Chromatin Folding Revealed by AFM Observation of In Vitro Reconstituted Chromatin. Scanning Near-Field Optical/Atomic Force Microscopy as a Tool for Simultaneous Specification of Chromosome Topography and Particular Gene Location on the Nanometer Scale. Isolation of Human and Plant Chromosomes as Nano-Materials. Proteome Analysis of Human Metaphase Chromosomes. Anti-Peptide Antibodies for Examining the Conformation and Molecular Assembly of an Intracellular Protein. Structure and Interactions of the Imitation SWI-Type Chromatin-Remodeling Complex, ATP-Dependent Chromatin-Assembly Factor. Dynamic and Functional Analysis of Chromosomal Proteins. Development of a Sustainable Chromosome Imaging Database. Image Database and Image Analysis of Chromosome Information.
