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Zusatztext "...a very clear account of the new approaches to generating human antibodies. It compares and contrasts conventional hybridoma technology with the more recent approaches! enabling the reader to assess the impact and usefulness of the different technique Informationen zum Autor Chief Hospital Scientist and Professor of Immunology, Flinders Medical Centre and Flinders University, Adelaide, Australia. Klappentext A review of what needs to be done to realise the potential of monoclonal antibodies. The book assesses the competing technologies with advice on the best approach for a particular situation. Zusammenfassung A review of what needs to be done to realise the potential of monoclonal antibodies. The book assesses the competing technologies with advice on the best approach for a particular situation. Inhaltsverzeichnis Contributors -- Abbreviations -- Preface -- Safety note -- 1. Introduction. H. Zola -- Introduction: the purpose of this book -- Monoclonal antibodies: scope and limitations -- Antibodies as laboratory reagents: a brief perspective -- Antibodies as therapeutic agents: a brief perspective -- Conclusions -- References -- 2. Making and using 'conventional' mouse monoclonal antibodies. P.J. Donohoe, P.J. Macardle and H. Zola -- Introduction -- Tissue culture -- Materials -- Working solutions -- The screening assay -- Immunization -- The myeloma cell line -- Fusion protocol -- Feeder cells -- Post-fusion care -- Mycoplasma -- Cloning -- Cryopreservation -- Thawing cells out -- Ascites fluid -- Production of monoclonal antibodies in dialysis tubing -- Continuous perfusion culture systems -- Purification -- Conjugation -- References -- 3. Human monoclonal antibodies from immortalized B cells. A. W. Hohmann, L. Spatz, M. Irigoyen and A. Manheimer-Lory -- Introduction -- Immortalized B cells to probe the human antibody repertoire -- Infectious diseases -- Human immunodeficiency virus -- Erythrocyte antigens -- Human histocompatibility antigens -- Tumor antigens -- Autoantibodies -- Immortalization of B cells with EBV -- EBV transformation of B cells: methodology -- Making human hybridomas -- Hybridoma methodology -- References -- 4. Reconstruction of monoclonal antibodies by genetic engineering. J.R. Adair and D.J. King -- Introduction -- The reconstruction process -- The binding site -- The effector functions -- Reconstructed antibodies in practice -- Summary and conclusions -- References -- 5. The genetic engineering of antibody constructs for diagnosis and therapy. G.A. Pietersz and l.F.C. McKenzie -- Introduction -- Principles of use of antibody conjugates -- Recent advances in immunoconjugate construction -- Recombinant antibodies and fusion proteins -- Use of cytokines and growth factors for targeting -- Potential problems -- Conclusions -- References -- 6. Superseding hybridoma technology with phage display libraries. R. Irving and P. Hudson -- Introduction -- Affinity maturation -- Discussion -- References -- 7. Construction and application of libraries of artificial antibodies. W. Ito and Y. Kurosawa -- Introduction -- Expression in E. coli of antibodies whose V genes are derived from V" and V L genes expressed in animals -- Characteristics of phage display antibodies -- Various devices for the generation of artificial antibodies -- Construction of libraries of completely synthetic antibodies -- Application of libraries of artificial antibodies -- Future perspectives -- References -- 8. Expression of antibody genes in mammalian cells. C. Bebbington -- Introduction -- Transient expression -- Stable mammalian expression systems -- Other factors influencing expression in mammalian cells -- Choosing an expression system -- References -- 9. Structure and application of single-chain Fvs as diagnostic and therapeutic agents. P. Hudson -- Introduction -- Design of single-chain Fv fragments -- Construction of scFv genes -- The structure of scFv fragmen...
