Immobilization of Enzymes and Cells

Enzymes and whole cells are able to catalyze the most complex chemical processes under the most benign experimental and environmental conditions. In this way, enzymes and cells could be excellent catalysts for a much more sustainable chemical industry. However, enzymes and cells also have some limitations for nonbiological applications: fine chemistry, food chemistry, analysis, therapeutics, and so on. Enzymes and cells may be unstable, difficult to handle under nonconventional conditions, poorly selective toward synthetic substrates, and so forth. From this point of view, the transformation-from the laboratory to industry-of chemical processes catalyzed by enzymes and cells may be one of the most complex and exciting goals in biotechnology. For many industrial applications, enzymes and cells have to be immobilized, via very simple and cost-effective protocols, in order to be re-used over very long periods of time. From this point of view, immobilization, simplicity, and stabilization have to be strongly related concepts. Over the last 30 years, a number of protocols for the immobilization of cells and enzymes have been reported in scientific literature. However, only very few protocols are simple and useful enough to greatly improve the functional properties of enzymes and cells, activity, stability, selectivity, and related properties.

List of contents

Immobilization of Enzymes as the 21st Century Begins.- Immobilization of Enzymes.- Cross-Linked Enzyme Aggregates.- Immobilization-Stabilization of Enzymes by Multipoint Covalent Attachment on Supports Activated With Epoxy Groups.- Glutaraldehyde in Protein Immobilization.- Practical Protocols for Lipase Immobilization Via Sol-Gel Techniques.- Encapsulation of Enzymes Using Polymers and Sol-Gel Techniques.- Design of Smart Biocatalysts.- Affinity Immobilization of Tagged Enzymes.- Bioaffinity Immobilization.- One-Step Purification, Immobilization, and Stabilization of Poly-Histidine-Tagged Enzymes Using Metal Chelate-Epoxy Supports.- Stabilization of Multimeric Enzymes Via Immobilization and Further Cross-Linking With Aldehyde-Dextran.- Purification, Immobilization, Hyperactivation, and Stabilization of Lipases by Selective Adsorption on Hydrophobic Supports.- Immobilization and Stabilization of Proteins by Multipoint Covalent Attachment on Novel Amino-Epoxy-Sepabeads®.- Improved Stabilization of Chemically Aminated Enzymes Via Multipoint Covalent Attachment on Glyoxyl Supports.- Stabilization of New Imprint Property of Glucose Oxidase in Pure Aqueous Medium by Cross-Linked-Imprinting Approach.- Reversible Covalent Immobilization of Enzymes Via Their Thiol Groups.- Very Strong But Reversible Immobilization of Enzymes on Supports Coated With Ionic Polymers.- Immobilization of Enzymes on Magnetic Particles.- Immobilization of Proteins on Gold Surfaces.- Immobilization of Enzymes on Electrodes.- Immobilization of Enzymes for Use in Organic Media.- Immobilization of Enzymes for Use in Ionic Liquids.- Immobilization of Enzymes for Use in Supercritical Fluids.- Immobilized Enzymes for Biomedical Applications.- Characterization of Immobilized Enzymes by Microcalorimetry.- Useof Immobilized Biocatalysts in Fluidized Bed Format.- Taylor-Couette Vortex Flow in Enzymatic Reactors.- A Novel Immobilization Method for Entrapment: LentiKats®.- Encapsulation of Cells in Alginate Gels.- Immobilization of Cells on Polyurethane Foam.- Immobilization of Cells With Transition Metal.- Immobilization of Microalgae.- Bioluminescence in Immobilized Cells for Biomass Detection and Biosensor Applications.- A Proteomic Approach to Biofilm Cell Physiology.- Encapsulation of Bacteria for Biodegradation of Gasoline Hydrocarbons.- Biomedical Applications of Immobilized Cells.

Summary

Enzymes and whole cells are able to catalyze the most complex chemical processes under the most benign experimental and environmental conditions. In this way, enzymes and cells could be excellent catalysts for a much more sustainable chemical industry. However, enzymes and cells also have some limitations for nonbiological applications: fine chemistry, food chemistry, analysis, therapeutics, and so on. Enzymes and cells may be unstable, difficult to handle under nonconventional conditions, poorly selective toward synthetic substrates, and so forth. From this point of view, the transformation—from the laboratory to industry—of chemical processes catalyzed by enzymes and cells may be one of the most complex and exciting goals in biotechnology. For many industrial applications, enzymes and cells have to be immobilized, via very simple and cost-effective protocols, in order to be re-used over very long periods of time. From this point of view, immobilization, simplicity, and stabilization have to be strongly related concepts. Over the last 30 years, a number of protocols for the immobilization of cells and enzymes have been reported in scientific literature. However, only very few protocols are simple and useful enough to greatly improve the functional properties of enzymes and cells, activity, stability, selectivity, and related properties.

Additional text

"Many well-known authors from research laboratories worldwide have contributed to this successful volume. . .this book provides a basic reference tool for all those who will need to immobilize biological materials in their work-not only for the beginner in the field, but also for experienced researchers."-Applied Biochemistry and Biotechnology

From Reviews of the First Edition...

"...enables researchers to rapidly enter the world of immobilization...an excellent reference work that will have long lasting value." -Quarterly Review of Biology

"...one of the most comprehensive collections of immobilization methodologies...[will] find extensive use as a methodology/reference text in any biotechnology-based laboratory." -Microbiology Today

"...a valuable tool for researchers in the many areas requiring enzyme and cell immobilizations." - Trends in Biotechnology

"...an ideal reference book to have around the laboratory." -Trends in Cell Biology

"...a worthwhile addition to any research library...especially useful for anyone involved in preparative isolations or needing immobilized reagents for specific research applications." -Doody's Health Sciences Book Review Journal

"...the coverage of the various techniques is excellent..." -The Analyst

Report

"Many well-known authors from research laboratories worldwide have contributed to this successful volume. . .this book provides a basic reference tool for all those who will need to immobilize biological materials in their work-not only for the beginner in the field, but also for experienced researchers."-Applied Biochemistry and Biotechnology

From Reviews of the First Edition...

"...enables researchers to rapidly enter the world of immobilization...an excellent reference work that will have long lasting value." -Quarterly Review of Biology

"...one of the most comprehensive collections of immobilization methodologies...[will] find extensive use as a methodology/reference text in any biotechnology-based laboratory." -Microbiology Today

"...a valuable tool for researchers in the many areas requiring enzyme and cell immobilizations." - Trends in Biotechnology

"...an ideal reference book to have around the laboratory." -Trends in Cell Biology

"...a worthwhile addition to any research library...especially useful for anyone involved in preparative isolations or needing immobilized reagents for specific research applications." -Doody's Health Sciences Book Review Journal

"...the coverage of the various techniques is excellent..." -The Analyst

Product details

• Assisted by: Jose M. Guisan (Editor), José M Guisán (Editor), José M. Guisán (Editor)
• Publisher: Springer, Berlin
• Languages: English
• Product format: Hardback
• Released: 17.04.2009
• EAN: 9781588292902
• ISBN: 978-1-58829-290-2
• No. of pages: 450
• Weight: 1 g
• Illustrations: XIV, 450 p. 217 illus.
• Series: Methods in Biotechnology; Methods in Biotechnology
• Subject: Natural sciences, medicine, IT, technology > Biology > Biochemistry, biophysics