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This volume details techniques, methods, and information relevant to the conservation of plant germplasm. Chapters guide researchers through conservation protocols, in vitro conservation, cryopreservation, seed conservation, and plant genetic resources. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Cutting-edge and comprehensive, Conservation of Plant Genetic Resources aims to provide efficient protocols for plant conservation.
List of contents
Overview of plant genetic resources conservation.- Strategies for the conservation of plant genetic resources.- In situ conservation of agricultural biodiversity and generation of projects in ejidos and communities in Mexico.- Germination protocol for cedar walnut seeds (Juglans pyriformis Liebm.) using thermal shock.- Evaluation and short-term conservation of Thrinax radiata (Arecaceae) seeds.- Short-term conservation and exploitation of sweet acacia (Vachellia farnesiana L. Wight & Arn.) through an efficient scarification-based germination protocol.- Germination of Calibanus hookeri (Lem.) Trel. seeds through imbibition in distilled water plus physical scarification.- Osmotic conditioning of amaranth seeds to increase germination.- In vitro propagation of Limonium cordatum (L.) Mill. for short-term conservation.- Uses and conservation of chaya in the southeast of Mexico.- Plant tissue culture: A short-term conservation tool .- Ex situ conservation of Opuntia spp. germplasm in a complementary agriculture model.- Ex-situ conservation of epiphytic orchids: A bibliometric review.- Medium-term preservation by the in vitro minimum growth method of Cedrela odorata L..- In vitro preservation of vanilla (Vanila planifolia Jacks. Ex Andrew) by slow growth.- Medium-term preservation by the in vitro minimum growth method of
