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This second edition volume expands on the previous edition with new methods and refinements ranging from tissue clearing and three-dimensional imaging, to explant culture systems, lineage tracing, high-resolution transcriptomics, metabolism, and chromatin structure analysis. The chapters in this book are organized by molecular depth, progressing from foundational methods to approaches that probe cellular and nuclear mechanisms. The protocols presented here reveal how cellular behaviors and molecular pathways are coordinated to shape the embryo. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and cutting-edge, Mouse Embryogenesis: Methods and Protocols, Second Edition is a valuable resource for both expert and novice researchers who are looking to learn more about this advancing field. The methods discussed in this volume will inspire new questions and experimental strategies to further advance our understanding of mammalian development.
List of contents
Mouse Genotyping.- Dynamic Imaging of Mouse Embryos and Cardiodynamics in Static Culture.- 3D Imaging of Gene Expression Domains in the Mouse Heart.- Fetal Mouse Heart Clearing, Imaging, and 3D Reconstruction.- Visualization of the Embryonic Great Vessels Using Plastic Resin Injection.- Explant Culture for Studying Lung Development.- Embryonic Gut Explant Model for Enteric Neural Crest Cells Migration.- Embryonic Gut Enteric Neural Crest Cells Derived Neurospheres.- Preparation of Mouse Embryonic Eye Sections for Routine Histology.- Embryonic Mouse Cardiac Fibroblasts Isolation.- Standardized Isolation, Culture, and Imaging of Contracting Cardiomyocytes from Neonatal Mouse Hearts.- Isolation and Culture of Neonatal Mouse Atrial and Ventricular Myocytes.- MicroRNA Quantification on Fast and Slow-Twitch Skeletal Muscles of Mouse.- Gene Expression Analysis on Single Myofibers from Mouse Edl and Soleus Muscles.- Characterization of Adipocyte Precursor Cells (APCs) by Lineage Tracing, Flow Cytometry, and Cytometry Time of Flight (CyTOF).- Investigating Protein Interactions in Single Cells by Proximity Ligation Assay (PLA).- Metabolic Plasticity in Embryogenesis Throughout the Lens of NAD+.- 3D Chromatin Architecture During Early Development: New Methods and New Findings.
