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This book aims to summarize the knowledge of smart nanoparticles in advancing plant gene transfer, focusing on their capacity for penetrating plant cells and delivering genetic engineering cargo such as nucleic acids, and eventually to enhance the efficiency and precision of plant genetic engineering. Plant gene transfer is a fundamental biotechnology for obtaining genetically modified plants. For decades, certain protocols have been developed to achieve successful plant genetic engineering events; however, there are still some limitations, particularly in the way to enhance their efficiency and precision. This book comprehensively discusses the benefits and limitations of nano-biotechnology on plant gene transfer, covering carbon, metallic, silicon, and bioinspired nanoparticles. This book presents the edge knowledge of nanocarriers in plant genome editing, including their methods and applications, particularly, which includes the part of CRISPR/Cas9 technology. From fundamentals to practical applications of nano-biotechnology, this book will be an ideal reference for readers in the fields of plant genetics, plant physiology, plant biotechnology, plant breeding, and materials science.
List of contents
Introduction to Plant Nano-Biotechnology: Advances and Prospects.- Nanoparticles in Plant Sciences: Types, Synthesis and Applications.- Biomolecule-Nanocarriers in Plant Biotechnology: Methods and Applications.- Nanoparticle Transportation in Plants: Mechanistic Insights and Applications.- Plant-Nanoparticle Interactions: Molecular Insights and Future Directions.- Nanoparticle-Mediated Nucleic Acid Delivery in Plant Cells: Molecular Insights and Applications.- Nanoparticles in Plant Regeneration: Methods and Applications.- Plant Genetic Engineering: Fundamentals and Technical Advancements.- Plant Genome Editing: Fundamentals and Technical Advancements.- Nanoparticles for Advancing Plant Gene Transfer: Opportunities and Challenges.- Nanoparticles for Advancing CRISPR/Cas9-Mediated Plant Genome Editing: Opportunities and Challenges.- Carbon Nanoparticles in Plant Genetic Engineering.- Metallic Nanoparticles in Plant Genetic Engineering.- Silicon Nanoparticles in Plant Genetic Engineering.- Nanoparticles for Organelle-Targeted Gene Delivery.- Nano-delivering RNAi in Plants: Opportunities and Challenges.- Ethical Consideration and Limitations of Nano-delivering Genetic Engineering in Agriculture and Food Systems.
About the author
Jen-Tsung Chen is a Professor of Cell Biology at the Department of Life Sciences, National University of Kaohsiung, Taiwan, where he teaches courses on cell biology, genomics, proteomics, plant physiology, and plant biotechnology. His research interests include bioactive compounds, chromatography techniques, plant molecular biology, plant biotechnology, bioinformatics, and systems pharmacology. In 2023 and 2024, Elsevier and Stanford University recognized Dr. Chen as one of the “World's Top 2% Scientists”. In 2025, Dr. Chen received the "Springer Nature Editorial Contribution Award" for his contributions to Plant Methods.
Summary
This book aims to summarize the knowledge of smart nanoparticles in advancing plant gene transfer, focusing on their capacity for penetrating plant cells and delivering genetic engineering cargo such as nucleic acids, and eventually to enhance the efficiency and precision of plant genetic engineering. Plant gene transfer is a fundamental biotechnology for obtaining genetically modified plants. For decades, certain protocols have been developed to achieve successful plant genetic engineering events; however, there are still some limitations, particularly in the way to enhance their efficiency and precision. This book comprehensively discusses the benefits and limitations of nano-biotechnology on plant gene transfer, covering carbon, metallic, silicon, and bioinspired nanoparticles. This book presents the edge knowledge of nanocarriers in plant genome editing, including their methods and applications, particularly, which includes the part of CRISPR/Cas9 technology. From fundamentals to practical applications of nano-biotechnology, this book will be an ideal reference for readers in the fields of plant genetics, plant physiology, plant biotechnology, plant breeding, and materials science.
