Read more
This volume provides readers with a detailed collection of in vitro and in vivo protocols developed to study inflammation and the interactions between host and micro-organisms. The chapters in this book cover a range of biological systems and methodological approaches such as direct inoculation of pathogenic yeasts into mouse lung by trans-tracheal instillation; murine models of experimental periodontal disease; quantitative in vitro and in vivo assays to assess the efferocytic efficiency of macrophages; murine models of alphavirus infection to study the immunopathogenesis of pain; and in vivo preclinical platforms to study coronavirus infection. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Cutting-edge and thorough, Models in Inflammation and Immunopharmacology is a practical and conceptual resource for researchers studying inflammation and host-microorganism interactions. This book is also a valuable reference for educators, clinicians, and policymakers who want to understand the foundations of experimental design in biomedicine.
List of contents
Handling and Experimentation with Germ-Free Mice.- Modeling Gut Barrier Dysfunction and Subsequent Opportunistic Infection by Candida albicans during Chemotherapy-Induced Intestinal Mucositis in Mice.- Direct Inoculation of Pathogenic Yeasts into Mouse Lung by Trans-Tracheal Instillation.- Murine Models of Experimental Periodontal Disease.- Infectious Models in Mice for Study of Gut Inflammation: Primary Cultures of Myenteric Neurons.- Intravital Microscopy of Mesenteric and Brain vessels: A Valuable Tool for Leukocyte Recruitment Evaluation.- Classical Histological Procedures with Murine Tissues.- Measurement of MPO, NAG, and EPO as an Indirect Quantification of Leukocyte Infiltration in Mouse Tissues.- Bridging Immunity and Hemostasis: Technical Foundations of Platelet-Leukocyte Co-Cultures.- Quantitative In Vitro and In Vivo Assays to Assess the Efferocytic Efficiency of Macrophages.- Physical Exercise as a Model for Investigation of the Inflammation Response in Mice.- Humanized Murine Model with Peripheral Blood Mononuclear Cells as a Strategy to Translate Experimental Biomedical Research.- Murine Models of Alphavirus Infection to Study the Immunopathogenesis of Pain.- Platforms to Evaluate Flavivirus-Induced Immunoinflammatory Responses and Their Pathogenesis.- Methods for Evaluating Neurotropic Viral Infections: Exploring Zika and Coronavirus Diseases.- In Vivo Preclinical Platforms to Study Coronavirus Infection.
