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Informationen zum Autor ISABELLA DALLE-DONNE , PHD, is Assistant Professor in the Department of Biology at the University of Milan, Italy. She has a PhD in cellular and molecular biology from the University of Milan. ANDREA SCALONI , PHD, is First Investigator at the Proteomics & Mass Spectrometry Laboratory, ISPAAM, National Research Council in Naples, Italy. He received his PhD in chemical sciences from the University of Rome "La Sapienza" in Italy. D. ALLAN BUTTERFIELD , PHD, is Alumni Professor of Chemistry and Director of the University of Kentucky Center of Membrane Sciences in Lexington, Kentucky, USA. He received his PhD in physical chemistry from Duke University. Klappentext Methodology and applications of redox proteomics The relatively new and rapidly changing field of redox proteomics has the potential to revolutionize how we diagnose disease, assess risks, determine prognoses, and target therapeutic strategies for people with inflammatory and aging-associated diseases. This collection brings together, in one comprehensive volume, a broad array of information and insights into normal and altered physiology, molecular mechanisms of disease states, and new applications of the rapidly evolving techniques of proteomics. Written by some of the finest investigators in this area, Redox Proteomics: From Protein Modifications to Cellular Dysfunction and Diseases examines the key topics of redox proteomics and redox control of cellular function, including: The role of oxidized proteins in various disorders Pioneering studies on the development of redox proteomics Analytical methodologies for identification and structural characterization of proteins affected by oxidative/nitrosative modifications The response and regulation of protein oxidation in different cell types The pathological implications of protein oxidation for conditions, including asthma, cardiovascular disease, diabetes, preeclampsia, and Alzheimer's disease Distinguished by its in-depth discussions, balanced methodological approach, and emphasis on medical applications and diagnosis development, Redox Proteomics is a rich resource for all professionals with an interest in proteomics, cellular physiology and its alterations in disease states, and related fields. Zusammenfassung Methodology and applications of redox proteomics The relatively new and rapidly changing field of redox proteomics has the potential to revolutionize how we diagnose disease! assess risks! determine prognoses! and target therapeutic strategies for people with inflammatory and aging-associated diseases. Inhaltsverzeichnis Preface xxiii Contributors xxvii Part I Oxidatively Modified Proteins and Proteomic Technologies 1 1 Chemical Modification of Proteins by Reactive Oxygen Species 3 Earl R. Stadtman and Rodney L. Levine 1.1 Introduction 3 1.2 Peptide Bond Cleavage 5 1.3 ß-Scission 6 1.4 Oxidation of Amino Acid Residue Side Chains 7 2 The Chemistry of Protein Modifications Elicited by Nitric Oxide and Related Nitrogen Oxides 25 Douglas D. Thomas, Lisa Ridnour, Sonia Donzelli, Michael Graham Espey, Daniele Mancardi, Jeffery S. Isenberg, Martin Feelisch, David D. Roberts, and David A. Wink 2.1 Introduction 25 2.2 Chemical Biology of NO 26 2.3 Chemistry of Metabolite Formation 40 3 Mass Spectrometry Approaches for the Molecular Characterization of Oxidatively/Nitrosatively Modified Proteins 59 Andrea Scaloni 3.1 Introduction 59 3.2 Mass Spectrometry Analysis of Oxidatively/Nitrosatively Modified Proteins 61 3.3 Proteomic Strategies for the Identification of ROS/RNS Protein Targets in Biological Matrices 76 3.4 Conclusions 84 4 Thiol-Disulfide Oxidoreduction of Protein Cysteines: Ol...
