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This research evaluated the production of Pleurotus ostreatus using coffee residues as an agroecological valorization alternative in the community of Shucush, Amazonas. Three residues derived from the coffee production cycle were selected: pulp, husk and concho, with which 16 substrate combinations were formulated in different proportions. The experimental design was completely randomized, covering 48 experimental units. The yield and earliness of P. ostreatus in each formulation were analyzed. The results revealed significant differences (p < 0.0001) between formulations. Formulation F03 (100% concho) recorded the highest yield with 19.73% and a moderate earliness of 17 days. The combination F08 (25% pulp, 75% concho) showed an optimal balance between yield (15.1%) and earliness (14 days). Formulation F11 (25% pulp, 50% husk, 25% concho) stood out for its rapid production, reaching 14.35% yield in 15 days. It was observed that formulations with high proportions of concho (75-100%) favored growth, while high proportions of pulp and husk inhibited production.
