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This volume details techniques involved in the study of endosymbionts. Chapters divided into four sections detail isolation, maintenance of diverse endosymbionts, classical microscopy, staining, PCR, FISH, flow cytometry, next-generation sequencing, endosymbionts, metabolites, and bacterial endosymbionts in insects. Step-by-step protocols are complemented by personal notes, precautions, and specifying the reagents to be used in each step to ensure the repeatability of the procedure across labs.
Authoritative and cutting-edge, Endosymbionts aims to empower researchers to expand the frontiers of endosymbiosis research and unlock new possibilities for science.
List of contents
Isolation and maintenance of endohyphal symbionts from the fungi.- Isolation and maintenance of algal endosymbionts from ciliates.- Isolation and maintenance of methanogenic endosymbionts.- Isolation and maintenance of cyanobacterial endosymbionts.- Isolation of fungal endosymbionts.- Isolation and Characterisations of Endosymbionts from Slime Mold Fruiting Bodies.- Isolation of Endosymbiotic Actinobacteria.- Isolation of Endosymbionts from Green Algae.- Isolation and identification of culturable endosymbiotic bacteria from insects.- Isolation and maintenance of endosymbionts from mycorrhiza.- Isolation and maintenance of endosymbionts from lichens.- Microscopy and staining-based identification of endosymbionts.- PCR based approaches to study endosymbionts.- Non-PCR Based Approaches to Study Endosymbionts.- Single cell-based technologies to study endosymbionts: Raman microspectroscopy.- Next generation sequencing approaches to study endosymbionts.- Screening of endosymbionts for plant growth promoting metabolites.- Screening of endosymbionts for EPS production for enhancement of symbiotic proficiency with the host.- Screening of Endosymbionts for Biocontrol Properties.- Screening of endosymbionts for virucidal activity.- Screening of endosymbionts for bactericidal activity.- Screening of endosymbionts for anticancer compounds.- Screening of Endosymbionts for Nematicidal Activity.- Screening of endosymbionts for biosurfactant production.- Screening of endosymbionts as anthelmintic agent.- Screening of endosymbionts for antibiotic and probiotic activity.- Diagnostic PCR to Detect Infection of Bacterial Endosymbionts in Insects.- Fluorescent in situ
Summary
This volume details techniques involved in the study of endosymbionts. Chapters divided into four sections detail isolation, maintenance of diverse endosymbionts, classical microscopy, staining, PCR, FISH, flow cytometry, next-generation sequencing, endosymbionts, metabolites, and bacterial endosymbionts in insects. Step-by-step protocols are complemented by personal notes, precautions, and specifying the reagents to be used in each step to ensure the repeatability of the procedure across labs.
Authoritative and cutting-edge,
Endosymbionts
aims to empower researchers to expand the frontiers of endosymbiosis research and unlock new possibilities for science.
