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This volume explores the latest developments in the study and application of phage biology. The chapters in this book are divided into five parts and cover topics such as phage display, selection, and evolution; genetic and chemical modification of phages; analyzing structures by electron microscopy; characterizing phage transcripts and proteins; and the biology of whole phages. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Cutting-edge and comprehensive, Phage Engineering and Analysis: Methods and Protocols is a valuable resource that will help both expert and novice researchers - from backgrounds ranging from microbiologists to biophysicists to chemical engineers - further enhance their understandingof this important and evolving field.
List of contents
Protocols for Building and Producing High Diversity Peptide Phage Display Libraries.- Generation of a Naïve Human scFv Phage Display Library and Panning Selection.- Structure-Guided and Phage-Assisted Evolution of Therapeutic Antibodies to Reverse On-Target Point Mutation-Mediated Resistance.- Combining a Base Deaminase Mutator with Phage-Assisted Evolution.- Understanding the Structural Requirements of Peptide-Protein Interaction and Applications for Peptidomimetic Development.- Contracting the Host Range of Bacteriophage T7 Using a Continuous Evolution System.- Single-Cell Proteomics by Barcoded Phage-Displayed Screening via an Integrated Microfluidic Chip.- Targeted Genome Editing of Virulent Pseudomonas Phages using CRISPR-Cas3.- Preparation of Bioconjugates of Chimeric M13 Phage and Gold Nanorods.- Liquid Glycan Array.- Electron Microscopy Methods for Phage-Based Study.- Visualization of Engineered M13 Phages Bound to Bacterial Targets by Transmission Electron Microscopy.- Purification of Single-Stranded RNA Bacteriophages and Host Receptors for Structural Determination using Cryo-Electron Microscopy.- Obtaining Detailed Phage Transcriptomes using ONT-Cappable-Seq.- Multi-Disciplinary Methods for Screening Toxic Proteins from Phages and Their Potential Molecular Targets.- CRISPRpi: Inducing and Curing Prophage using CRISPR-Interference.- Isolation of Bacteriophages on Actinobacteria Hosts.
