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This volume explores the latest advancements and techniques used by researchers to work with plant parasitic nematodes (PPNs) and to study their interactions with crop plants. The chapters in this book cover topics such as ways to obtain samples from soil and different plant tissues; rearing nematodes in greenhouse setting; methods used to axenically rear nematodes in vitro; Denaturing Gradient Gel Electrophoresis (DGGE) separation; methods for ROS detection and hydrogen peroxide quantification; methods to elicit plant resistance against nematodes; methods for the detection of Pathogenesis Related (PR) genes that are the executioners of immunity in plant-RKN interactions; and laboratory procedures that test nematodes on their isolation, count, culture, identification, pathogenicity, virulence, and environmental tolerance. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Cutting-edge and thorough, Plant-Nematode Interactions is a valuable tool for any researcher interested in learning more about this interesting and developing field.
List of contents
Sampling and Extraction of Plant-Parasitic Nematodes.- Rearing and Conservation of Nematode Populations under Greenhouse or Axenic Conditions.- Detection of Resistance, Susceptibility, Tolerance, and Virulence in Plant-Nematode Interactions - Part I: Sedentary Endoparasitic Nematodes.- Detection of Resistance, Tolerance, and Virulence in Plant-Nematode Interactions - Part II: Migratory and Semi-Endoparasitic Nematodes.- Molecular Techniques for Root-Knot Nematode Identification.- Use of PCR-DGGE Based Molecular Methods to Analyse Nematode Community Diversity.- Investigations on Microbes Attached to the Cuticle of Phytonematodes.- Detection of the Effects of Root Exudates (Diffusates) on Nematode Hatching and Attraction.- Use of Chemicals and Biological Control Agents as Activators of Plant Defense against Sedentary Endoparasitic Nematodes.- ROS Detection and Quantification in Plant-Nematode Interactions.- Detection of PathogenesisRelated (PR) Genes in Susceptible Tomato Plants Infected by Meloidogyne spp..- Conceptual Framework of Epigenetic Analyses of Plant Responses to Sedentary Endoparasitic Nematodes.- Heat Shock Proteins as Potential Indicators of Induced Stress in Nematodes.- Reproduction of Entomopathogenic Nematodes to Use in Pest Control.
