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This volume details critical and valuable experimental protocols to facilitate researchers in this fascinating field. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.
Authoritative and cutting-edge, Maternal Placental Interface: Methods and Protocols aims to ensure successful results in the further study of this vital field.
List of contents
Histological analysis of trophoblast cells in the mouse placental labyrinth zone.- Isolation and Immunophenotyping of Leukocytes from the Human Maternal-Fetal Interface.- Evaluation of leukocyte chemotaxis induced by human fetal membranes in an in vitro model.- In vitro culturing of Human Term Placental Explants.- In vitro culturing of Human Trophoblasts from Term Placenta.- Culture of human fetal membranes in a two independent-compartment model: an ex vivo approach.- Isolation of Primary Human Decidual Cells from the Fetal Membranes of Term Placentae.- Generation of knockout mouse trophoblast stem cells by CRISPR/Cas.- CRISPR-activation in mouse trophoblast stem cells.- Feto-maternal interface organ-on-chip: a new technology to study ascending infection.- Culture and Maintenance of Immune Cells to Model Innate Immune Status at the Feto-Maternal Interface.- Placental trophoblast cell isolation from the term placenta.- Dissociation of placental tissues for single-cell techniques.- Immunophenotyping of leukocytes in amniotic fluid.- Obtainig of tissue of human amniotic membrane and identification of pluripotency markers by immunofluorescence.- DNA isolation from fresh placenta, in formalin and paraffin.- Infection of fetal membranes with Mycobacterium tuberculosis and tissue processing to isolate RNA for expression analysis.
