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This volume details various aspects of the very final stages of mouse oocyte development, and very early embryo development. Chapters present methods ranging from in vitro growth of follicles, ewe oocytes, meiosis, identification of LADs (Lamin Associated Domains)/TADs (Topological Associated Domains), analysis of the oocyte, early embryo transcriptome, and mechanical characterization of these cells. Written in the highly successful Methods in Molecular Biology series format, the chapters include brief introductions to the material, lists of necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and a Notes section which highlights tips on troubleshooting and avoiding known pitfalls.
Authoritative and cutting-edge, Mammalian Oocyte Development: Methods and Protocols, aims to be comprehensive guide for researchers in the field.
List of contents
Isolation of sheep ovarian cortex and in vitro culture to study folliculogenesis.- Culture conditions for the development of equine oocytes.- In vitro maturation and time-lapse imaging of immature human oocytes: a direction towards clinical applications.- Collection of growing bovine oocytes and library preparation for single-cell RNA sequencing.- RNA-fluorescence in situ hybridization for the detection of RNA in mammalian oocytes and embryos.- Whole mount Oligopaint fluorescence in situ hybridization of mouse oocytes, eggs, or early-stage Embryos.- Optical coherence microscopy for oocyte imaging.- Electron microscopy of human oocytes.- Imaging and quantifying F-actin bundles in the cortex of mammalian oocytes.- Assessment of chromosome congression defects in mammalian oocytes.- Using photoactivatable GFP to study cytokinesis dynamics in early development.- Nuclear tracking during early embryonic divisions.- Highly multiplexed immunofluorescence imaging of mouse oocytes.- " JESS Simple western " - A new technology to assess protein abundance in mouse oocytes.- Machine learning methods to characterize mouse and human oocytes.- Design and use of a microfluidic device for oocyte quality screening.
Summary
Authoritative and cutting-edge, Mammalian Oocyte Development
